Parallel tests were conducted utilizing, the capillary tube migration test and the Boyden chamber assay, in order to determine whether the decrease in leukocyte chemotaxis that occurs if overoptimal cytotaxin concentrations are applied is due to migration inhibition. Overoptimal doses of casein produced decreased chemotactic response and migration inhibition for both rabbit macrophages and neutrophils. However, guinea pig neutrophils exhibited no decrease in chemotaxis despite high casein doses. Overoptimal doses of acid-denatured anaphylatoxin produced a decreased chemotactic response and migration inhibition of neutrophils. In both assays, this agent showed no effect upon macrophages. It is concluded that a chemotactic signal at different concentrations can elicit unidirectional migration or migration inhibition. Accordingly, chemotactic leukocyte attraction could be antagonistically regulated not only by serum-derived and lymphocyte-derived migration inhibitory factors but also by high doses of the chemotactic factor itself. Thus, the Boyden chamber technique can measure both chemotactic migration and migration inhibition phenomena.

This content is only available via PDF.
© 1977 S. Karger AG, Basel
1977
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.