Titres of E. coli O6 antibody obtained with the direct bacterial agglutination (DBA) technique, the indirect haemagglutination (IHA) technique and the ammonium sulphate precipitation (ASP) technique were compared using rabbit immune sera and IgG and IgM antibody fractions thereof, untreated as well as reduced with β-mercaptoethanol (ME). Poor rank correlation was obtained between the different assays on sera whether reduced or not. However, statistically significant correlation was obtained for the DBA and IHA titres on the IgM as well as IgG antibody fractions. There was also good correlation between the IHA and ASP titres on IgG antibody fractions. The DBA and IHA techniques both favoured IgM antibodies compared to the ASP method. This effect, however, was most accentuated by the IHA technique. In contrast, the IHA technique underestimated IgG antibodies more than the DBA technique. This could not be explained by influence of different antibody avidities, although the methods previously have been shown variously affected by such antibody properties. The study thus indicates an explanation for discrepancies in E. coli·antibody titres in patients with urinary tract infections obtained by different investigators using direct and indirect agglutination techniques or radioimmunoassay.

This content is only available via PDF.
© 1975 S. Karger AG, Basel
1975
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.