The stability of Ascaris suum allergen A to chemical and enzymatic agents has been studied. The allergenic activity measured in the rat passive cutaneous anaphylaxis test was found to be stable to heat at 100 °C, hot acid and periodate at 4 °C but to be destroyed completely by hot alkali. The allergen showed various degrees of resistance to treatment by the proteolytic enzymes trypsin, papain, chymotrypsin, elastase, subtilisin and pronase but was hydrolysed readily by pepsin. The effect of alkali on the allergen has been further studied by thin-layer gel filtration, ultracentrifugation, electrophoresis and amino acid analysis. Although the amino acid composition and sedimentation coefficients remained the same as the native allergen after this treatment, the isoelectric point and electrophoretic mobility were changed.

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© 1974 S. Karger AG, Basel
1974
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