In extending previous studies, it was shown that not only malignant but also normal murine lymphoid cells contain the heterophile antigen combining with infectious mononucleosis (IMS) sera. Evidence was presented, however, that malignant lymphoid cells contain heterophile antigen at a higher density and possibly in a more ‘complete’ form than normal lymphoid cells. Nonlymphoid nucleated murine cells contain very little, if any, heterophile antigen. Distribution of heterophile antigen in normal rat tissues is similar to that in normal murine tissues. Ox and sheep contain heterophile antigen not only on erythrocytes and lymphoid cells but also on nonlymphoid nucleated cells. Naturally occurring human antibodies to murine thymocytes differ in their serological specificity from heterophile antibodies of IMS sera.