Using rabbit anti-rabbit heart auto-antibodies, cultures of dissociated beating rat heart cells were examined for the presence of heart auto-antigens by immunofluorescence. Several patterns of staining of myocardial cells were observed. One type of auto-antibody revealed crisp myofibrillar cross-striations, consisting of wide bright segments often split by a thin dark band. Auto-antibody producing this pattern was potent for auto-antigen B. Rabbit anti-rabbit heart auto-antibodies rich in the auto-antigen D systems produced a granular staining in the myocardial cells, while an auto-antibody reactive with all five of the cardiac auto-antigens showed both granular and myofibrillar cross-striations. All the heart auto-antibody preparations reacted with cultured neonatal rat skeletal muscle cells to show diffuse cytoplasmic fluorescence and fine myofibrils. In all cases, rabbit anti-rabbit heart auto-antibodies failed to stain significantly the non-contractile cells present in the tissue cultures (‘endotheliod’, fibroblast, etc.). Sarcolemmal staining was not a prominent feature of these reactions. When the rabbit anti-rabbit heart auto-antibodies were exposed to living beating rat heart cells, no staining was observed, indicating the absence of these antigens on the cell surface.

This content is only available via PDF.
© 1973 S. Karger AG, Basel
1973
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.