(1) The properties of the 5 cardiac antigens in human heart which cross-react with rabbit anti-rabbit heart auto-antibodies were studied, and compared to the rabbit heart auto-antigens analyzed previously. They were found to be similar in many respects. All were rendered undetectable by proteinases, but were unaffected by ribonuclease or desoxyribonuclease; they were salted out by exposure to similar concentrations of ammonium sulfate or potassium phosphate; they were variably labile to heat or pH extremes; and their elution characteristics from hydroxyl apatite and Sephadex G-100 were similar. (2) Further evidence was obtained that the human cardiac proteins detectable with rabbit anti-rabbit heart auto-antibodies were found only in human heart, and were not present in human skeletal muscle, kidney, pancreas, liver or plasma. (3) Passive hemagglutination assay correlated with immunodiffusion tests in terms of organ specificity and auto-antibody titers. (4) The precipitin systems formed between rabbit anti-rabbit heart auto-antibodies and human heart extracts failed to show esterase, catalase, glucosaminidase, glucuronidase, cysteine-amino-peptidase or phosphatase activities, as had been found earlier for the rabbit heart auto-immune system. Cardiolipin was found to be unrelated to any of the above human cardiac auto-antigens. (5) Urea, with or without dithiothreitol, incactivated only 2 of the human cardiac auto-antigens.

This content is only available via PDF.
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.