... to determine the role of Rac2 in eosinophil mediator release and F-actin-dependent shape change in response to chemotactic stimuli. Methods: Rac2-deficient eosinophils from CD2-IL-5 transgenic mice crossed with rac2 gene knockout animals were examined for their ability to release superoxide through respiratory...

... as an anti-inflammatory agent. We investigated the effect of 14-membered ring macrolides, erythromycin and clarithromycin, on human neutrophil functions and endothelial cell damage induced by neutrophils. The superoxide production of neutrophils and Ca 2+ influx into neutrophils induced by TV-formyl...

... Activation Superoxide Original Paper Int Arch Allergy Immunol 1993;101:227-233 Philip Tagari Eve-Isabelle Pecheur Michael Scheid Pamela Brown Anthony W. Ford-Hutchinson Donald Nicholson Department of Pharmacology. Merck Frosst Centre for Therapeutic Research, Dorval, Canada Key Words Interleukin-5...

...Shoji Nagata; Donald K. Kebo; Steven Kunkel; Michael Glovsky The effect of adenylate cyclase activators on C5a- and f-Met-Leu-Phe-induced human neutrophil aggregation, enzyme release and superoxide production was investigated. C5a-stimulated superoxide production was markedly inhibited by adenylate...

..., cathepsin G and proteinase 3. Superoxide generation was quantitated with the cytochrome C reduction method. A panel of cytokines and chemokines in the culture supernatants were measured with a multiplex beads array system. Effects of an elastase inhibitor, sivelestat, and a serine protease inhibitor, PMSF...

... and in combination, on bronchial epithelial cell-mediated eosinophil superoxide production in vitro . Methods: We employed 2 experimental approaches. First, superoxide production by human eosinophils incubated with conditioned medium (CM) from human bronchial epithelial cells cultured for 24 h with vehicle, BUD...

... to enhance respiratory burst through the formyl peptide receptors. Objective: This study was undertaken to determine the intracellular signaling pathway involved in WKYMVm-stimulated superoxide production by human eosinophils. Methods: Purified eosinophils from peripheral blood were stimulated with various...

.... Methods: Purified eosinophils from the peripheral blood were incubated with various concentrations (0–200 µg/ml) of German cockroach extract antigen. Effector functions of eosinophils were checked by degranulation and superoxide anion production. In addition, we examined surface expression of CD11b...

... with a protein kinase C (PKC) inhibitor which generally inhibits PKC isoforms has been shown to modulate several eosinophil functions in distinct manners, in that PKC inhibition enhanced CD11b expression and cellular adhesion, but inhibited superoxide generation and degranulation in PAF-stimulated human...

... mediators. On the other hand, we found that the application of compound 48/80, a typical histamine liberator, generated superoxide in mast cells. In the present study, we investigated the mechanism of superoxide production in mast cells with respect to AA signaling in conjunction with a fine structural...

..., Tokuyama K, Morikawa A, Kuroume T, Barnes PJ: Platelet-activating factor-induced enhancement of superoxide anion generation in guinea pigs. Eur J Pharmacol 1993;232:7–12. 11. Nakamura M, Honda Z, Izumi T, Sakanaka C, Mutoh H, Minami M, Bito H, Sayama Y, Matsumoto T, Noma M, Simizu T: Molecular cloning...