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1-3 of 3
Keywords: Protein G
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Journal Articles
Subject Area:
Immunology and Allergy
Int Arch Allergy Immunol (1994) 104 (2): 204–206.
Published Online: 03 September 2009
...Zhikang Peng; Allan B. Becker; Estelle R. Simons We studied the binding of protein A and protein G to human monoclonal and polyclonal IgE. An ELISA was used to measure human IgE. Protein A-Sepharose did not bind to monoclonal IgE, but bound to 12–14% of serum polyclonal IgE and to ––3...
Journal Articles
Subject Area:
Immunology and Allergy
Yuko Kikuchi, Toshiro Takai, Mikiko Ota, Takeshi Kato, Ken Takeda, Kouichi Mitsuishi, Shigaku Ikeda, Ko Okumura, Hideoki Ogawa
Int Arch Allergy Immunol (2006) 141 (4): 322–330.
Published Online: 15 November 2006
... adsorbed to alum were used for the assays. Total IgE was measured by a sandwich enzyme-linked immunosorbent assay (ELISA). Allergen-specific IgE was assayed using plates coated with the allergens after the removal of IgG from sera with protein G-coupled sepharose beads in wells of other plates...
Journal Articles
Subject Area:
Immunology and Allergy
Yukio Kadooka, Tadashi Idota, Hiromi Gunji, Masaharu Shimatani, Hiroshi Kawakami, Shun-ichi Dosako, Tomoji Samori
Int Arch Allergy Immunol (2000) 122 (4): 264–269.
Published Online: 04 September 2000
... (IgG-IgE) in sera affect the assay. In this paper, we attempt to determine accurate specific IgE levels by selective removal of IgG with a protein G-immobilized gel (PG) and by acid treatment of the PG to compensate for the unintended removal of IgE, probably due to the PG binding IgG-IgE. Methods: IgG...
