... (in the absence of plant pathogenesis-related proteins [PR-10] or profilin sensitization). We used radar graphic display to highlight, at a glance, the molecular reactivity profiles associated with relevant disease-specific patterns. e.scala@idi.it 19 02 2020 13 03 2020 20 5 2020 ©...

... at molecular level into major and minor allergens. Clinical complaints are usually triggered by major allergens, while the role of sensitization to the panallergens profilin and polcalcin still remains unclear. Methods: Eighty-six patients from southern Bavaria with sensitization to the panallergens profilin...

... Verga; Paolo Maria Matricardi; for the Italian Pediatric Allergy Network (I-PAN) Background: Little is known about the prevalence and clinical relevance of hypersensitivity to the plant panallergen profilin in children. Objectives: The present study aimed to investigate prevalence, risk factors...

... to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Allergen-specific nasal challenge IgE antibodies, specific ImmunoCAP In vitro tests Latex allergy Profilin Skin prick test Latex allergy is a serious disease...

...R. Núñez; F. Carballada; M. Lombardero; L. Jimeno; M. Boquete Background: The role of profilin as a food allergen is well established, but little research has been done about its ability to elicit respiratory disease. Profilin is considered more of a confounding allergen on skin testing with whole...

...-specific IgE ratio <0.5 had more often IgE to Amb a 1, profilin, polcalcin and CCDs than subjects with a ratio >0.5. Conclusions: Mugwort-sensitized subjects have different IgE reactivity profiles to weed allergens, reflecting their exposure to various pollens. Subjects with a low ratio between...

...Bárbara Cases; Carlos Pastor-Vargas; Félix Gil Dones; Marina Perez-Gordo; Aroa S. Maroto; Manuel de las Heras; Fernando Vivanco; Javier Cuesta-Herranz Background: Plant profilins have been reported as minor allergens. They are a well-known pan-allergen family responsible for cross-reactivity...

...); (3) sensitization to purified allergens: Pru p 3, profilin, Pla a 1 – Pla a 2, Sal k 1, PR-10 proteins and Len c 1. Conclusion: Results showed relevant and significant differences between both groups of pollen-allergic patients depending on whether or not they suffered from plant-derived food allergy...

... by the cross-linking of IgE receptors through polyvalent allergens. A detailed understanding of the cell surface phenomena and the rational development of new therapies require high-resolution structural information. Methods : The structures of two widespread allergens, birch pollen profílin (BPP) and Phl p 2...

... identified as profilins. Isolation of the relevant allergens was made by affinity chromatography on a poly- L -proline -Sepharose column, followed by gel filtration and anion exchange chromatography in the micropreparative SMART® System. A significant but low antigenic cross-reactivity between M. annua...

..., immunoblotting with specific antibodies and immune electron microscopy. Pollen allergens such as the major birch pollen allergen, Bet v I, the major timothy grass pollen allergens, Phl p I and Phl p V, group-II/III allergens from timothy grass and profilins were released rapidly and in large amounts from...

...H.-P. Rihs; P. Rozynek; K. May-Taube; B. Welticke; X. Baur Highly degenerate profilin-specific primers were used to amplify by polymerase chain reaction the profilin-coding sequence of wheat cDNA. Analyzing the sequencing data after subcloning revealed the existence of three isoforms. The high...

...Ronald van Ree; Valentina Voitenko; Astrid van Leeuwen; Rob C. Aalberse Sera with IgE antibodies against grass pollen often contain IgE against vegetable foods. We investigated the role of the ubiquitous protein profilin in this cross-reactivity. Profilin was purified from Lolium perenne grass...

... (group V) and profilin from timothy grass, which when used together allowed the diagnosis of grass pollen allergy in 97 out of 98 tested grass pollen-allergic patients employing a simple plaque lift technique. This recombinant test based on plaque lifts containing allergen-β-galactosidase fusion proteins...

...Rudolf Valenta; Michael Duchêne; Susanne Vrtala; Peter Valent; Christian Sillaber; Fatima Ferreira; Margit Tejkl; Reinhold Hirschwehr; Christof Ebner; Dietrich Kraft; Otto Scheiner Recently we identified birch profilin as a novel pollen allergen which showed significant sequence homology...

...–120 kDa) were detected in watermelon extract. Three major allergens were identified as malate dehydrogenase (36 kDa), triose phosphate isomerase (28 kDa) and profilin (13 kDa). Purified allergens individually inhibited IgE binding to the whole watermelon extract. Conclusions: All in all these results...

... the specific serum IgE-binding profiles to individual P. judaica pollen recombinant major allergen, and Phleum pratense cytoskeletal profilin and a 2-EF-hand calcium-binding allergen homologous to cross-reactive Parietaria pollen allergens, in patients allergic to pollen with positive skin test towards...

... mimicking various types of wet surfaces, the time-dependent elution profiles of total protein, a cell wall-bound acid phosphatase activity (acPase), allergenic (profilin, Art v 1) and nonallergenic molecules (14-3-3 protein, actin) were monitored. Results: The release kinetics of total protein and cell wall...

...S.T.H.P. Bolhaar; R. van Ree; Y. Ma; C.A.F.M. Bruijnzeel-Koomen; S. Vieths; K. Hoffmann-Sommergruber; A.C. Knulst; L. Zuidmeer Background: Allergy to sharon fruit (persimmon) has been only rarely reported. Cross-reactivity with pollen (profilin and Bet v 6) appeared to be involved, but Bet v 1 has...

...Riccardo Asero; Gianni Mistrello; Daniela Roncarolo; Stefano Amato Background: In patients showing multiple reactivity to seasonal airborne allergens, a skin prick test (SPT) with Parietaria pollen scores frequently negative, suggesting that profilin from Parietaria might not share IgE-binding...