... and investigated the effect of such pre-exposure on subsequent oral administration of peanut. Methods: BALB/cJ mice were exposed to purified Ara h 1 or to a non-defatted roasted peanut extract (PE) by simple deposit of allergens solutions on non-altered skin or in the nostrils. Exposures were performed 6 times...

... examined eosinophil infiltration and the cytokine profiles of both airway and peripheral blood in antigen-sensitized mice infected with RSV to investigate the pathogenesis of exacerbations of asthma due to RSV infection. Methods: Ovalbumin (OVA)-sensitized mice were challenged by OVA inhalation 3 times...

...S. Vrtala; M. Grote; F. Ferreira; M. Susani; B. Stocker; D. Kraft; R. Valenta Recombinant tree pollen allergens (recombinant Bet v I and recombinant birch profilin, Bet v II) were purified and used to immunize BALB/c and B6D2F1 mice with Al(OH) 3 to elicit a specific IgE response. Serum from...

...Chun-Keung Yu; Bei-Chang Yang; Shiour-Ching Lee; Jiu-Yao Wang; Tzuen-Ren Hsiue; Huan-Yao Lei Dermatophagoides farinae (Der f) is one of the most common species of dust mites that induce asthma and allergic rhinitis. We have reported that Der f challenge on sensitized mice elicited a distinct type...

...Hans-Christian Schuppe; Johanna Kulig; Ulrich Kühn; Uwe Lempertz; Peter Kind; Jürgen Knop; Detief Becker The sensitizing properties of different complex salts of platinum were defined in vivo by means of the popliteal lymph node (PLN) assay in mice. Hexa- and tetrachloroplatinates were confirmed...

... understanding of the role played by the Stat3/IL-6 and Socs3 pathway in airway inflammation is lacking. We report the alteration in the status of expression and activation of Stat3 by ovalbumin (OVA), and establish its relationship with Socs3 and IL-6 in the lungs of mice with eosinophilic pulmonary...

... have suggested an important role for the usually constitutive neural NOS isoform (nNOS, or NOS 1). Aim: To investigate how NOS 1 may influence allergic inflammation, we used NOS 1 knockout mice and their wild-type (WT) controls. Methods: Mice were sensitized and challenged with ovalbumin (OVA) using...

.... Priorly established oral tolerance due to antigen in the diet may radically impact on the CT-adjuvantized immune response. The present study served to evaluate the effect of priorly established low-dose oral tolerance on the CT-adjuvantized immune response towards a food antigen. Methods: Mice fed a diet...

...Hanne R. Christensen; Susanne W. Bruun; Hanne Frøkiær Background: Soybean protein is used in a number of food products but unfortunately is also a common cause of food allergy. Upon ingestion of soy protein, healthy mice like other animals and humans generate a soy-specific antibody response...

...Masao Hirasawa; Hirofumi Deguchi; Akira Ukimura; Yasushi Kitaura Background: Eosinophilic myocarditis often occurs spontaneously in DBA/2 mice. Relationships between infiltrating eosinophils (Eos), T lymphocytes and interleukin-5 (IL-5) in this disorder were investigated microscopically...

... metabolisable and has been successfully employed in allergy vaccines for a number of years. Methods: Mice were immunised with MPL ® adjuvant in conjunction with separate preparations of either ovalbumin or glutaraldehyde-modified ragweed pollen extract both coprecipitated with L-tyrosine. The specific antibody...

... for the sensitizing capacity of the residual protein, a mouse IgE test was used to detect differences in the production of specific IgE by chemical or enzymatic preparations of the antibiotics. Methods: Balb/c female mice were immunized intraperitoneally with alum and conjugates of different amoxicillins...

... cell epitopes on Cry j 1 by using a synthetic peptide approach in mice. Methods: We synthesized 35 overlapping peptides that cover the entire length of Cry j 1 and examined whether mouse IgE and IgG antibodies produced by immunization with Cry j 1 reacted to the Cry j 1 peptides. Results and Conclusion...

...Berit Granum; Per Ivar Gaarder; Åse Eikeset; Berit A. Stensby; Martinus Løvik Background: We have previously reported that simple and well-characterised particles, such as polystyrene particles (PSP), have an IgE adjuvant effect in mice. The purpose of this study was to explore the importance...

... mice (CD1) and inbred mice (BALB/c, C3H, SJL, C57BL/6). In mice deficient for IgE or IgG1, T. spiralis only was used. In homologous conditions (i.e., same Trichinella species for antigens and infectious agent) mortality was very high (up to 100% in SJL and C57BL/6). The anaphylactic response...

...Masataka Korenaga; Yoko Akimaru; Yoshihisa Hashiguchi Treatment with recombinant interleukin–3 (rIL–3) augmented IL–4 production of spleen cells in mice infected with Trichinella spiralis. In a previous report, we showed that treatment with rIL–3 accelerated IgE responsiveness in mice. We have...

...Kazuteru Wada; Osamu Kaminuma; Akio Mori; Aya Nakata; Koji Ogawa; Hideo Kikkawa; Katsuo Ikezawa; Matsunobu Suko; Hirokazu Okudaira We have recently demonstrated that airway eosinophilic inflammation can be transferred to unprimed mice by infusion of IL-5-producing T cell clones. In this study, we...