... by inhibiting NLRP3 inflammasome activation in macrophages. Methods: Allergic mouse models were established by ovalbumin stimulation, and mice were treated with miR-146a-5p agomir and oe-TIRAP 3 h before OVA stimulation. The pathological changes of lung tissues were observed by hematoxylin-eosin staining...

...Li Wang; Xueyan Liu; Xicheng Song; Lei Dong; Dawei Liu Background: The aim of this study was to investigate the effect of MiR-202-5p on macrophages involved in allergic rhinitis. Methods: Thirty allergic rhinitis patients and 10 healthy controls were recruited. Nasal lavage was utilized to collect...

... at 2-week intervals. Lung pathology, bronchoalveolar lavage fluid (BALF) cytological profiles, and inflammatory mediators in BALF were investigated. Bone marrow-derived macrophages (BMDM) from TLR2 -/- ,TLR4 -/- , TLR2/4 -/- , TLR7/9 -/- ,MyD88 -/- , and WT C57BL/6J mice were stimulated with B. adusta...

... cell-bound IFN-γ retained functional activity since it could induce NO production by responder macrophages. IFN-γ-presenting function was inhibited by incubation of the mast cells with chondroitinases ABC and B but not AC nor heparinase [ 8 ]. We conclude that IFN-γ binds to mast cells via cell surface...

...Ann M. Dvorak; Peter F. Weller; Susan Harvey; Ellen S. Morgan; Harold F. Dvorak Subcellular fractions of purified cytoplasmic, nonmembrane-bound lipid bodies were prepared from [ 3 H]-arachidonic acid-labeled guinea pig peritoneal macrophages and line 10 hepatocarcinoma cells. These fractions...

... for the production of chemotactic lymphokine for eosinophils has a pI value of about 4–5, and that the activity for MCF production is detected in two fractions of pi about 5–6, and 7–8. Macrophage lineage cell line cells, such as THP-1 and U-937, also release similar factors after differential stimulation. 3 9...

.... Specialized morphological and biochemical studies have provided evidence for the involvement of lipid bodies in eicosanoid synthesis. Mast cell Macrophage Lipid bodies Arachidonic acid Cyclooxygenase Prostaglandin Ultrastructure Autoradiography Immunocytochemistry Subcellular fractionation...

... microspheres can efficiently induce apoptosis in human eosinophils, and second, whether or not these apoptotic eosinophils can be phagocytosed by macrophages without the release of granular proteins. Methods: Purified human eosinophils were treated with anti-CD30 mAb-coated polystyrene microspheres (diameter...

...Nadir S. Hirji; Tong-Jun Lin; Mark Gilchrist; Genevieve Nault; Osamu Nohara; Brock J. Grill; Mirodrag Belosevic; Grant R. Stenton; Alan D. Schreiber; A. Dean Befus Background: We previously identified, using flow cytometry and in situ RT–PCR, a novel CD8 molecule on rat alveolar macrophages (AM...

...Hiroshi Kato; Atsuko Horino; Maiko Taneichi; Naoyuki Fukuchi; Yuzuru Eto; Hiroshi Ushijima; Katsutoshi Komuro; Tetsuya Uchida We have previously reported that a lipid molecule in the membrane fraction of cloned macrophage hybridomas inhibited the growth of lymphocytes and several tumor cell lines...

...Hiroshi Kato; Atsuko Horino; Shingou Sakurai; Hiroshi Ushijima; Katsutoshi Komuro; Tetsuya Uchida We have previously reported that the growth of lymphocytes and tumor cells with lymphocyte lineage was strongly inhibited by a part of cloned macrophage hybridomas. This growth inhibition...