... patients were selected and skin prick tests (SPTs) were performed on them using commercial gluten, wheat flour extracts, prolamin fractions and a purified natural LMW-GS P42. The IgE-binding ability of natural and recombinant wheat prolamins was verified by immunoblot experiments. Cross-reactivity between...

... immunogenicity. Methods: IgE binding of sera of patients allergic to fresh and processed (smoked, salted/sugar-cured, canned, lye-treated and fermented) cod, haddock, salmon, trout, tuna, mackerel and herring and of hydrolysates based on salmon and whiting was investigated using immunoblot and inhibition ELISA...

... common IgE binding epitopes. 22 06 1994 4 04 1995 4 9 2009 © 1995 S. Karger AG, Basel 1995 Copyright / Drug Dosage / Disclaimer Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means...

... Grass pollen allergen IgE binding Immunoblotting Original Paper Int Arch Allergy Immunol 1995; 108:55-59 A. Petersen A. Bufe G. Schramm M. Schlaak W.-M. Becker Division of Allergology, Forschungsinstitut Börstel, Germany Characterization of the Allergen Group VI in Timothy Grass Pollen (Phi p 6) II...

... and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Monoclonal antibody Recombinant Fab IgE binding Major birch pollen allergen Bet v 1 IntenulkMul Arctmcs of Allergy...

... sulphate polyacrylamide gel electrophoresis and subjected to limited partial proteolysis and cyanogen bromide cleavage. The generated digests were visualised on gels by silver staining. Replicate gels were blotted and screened with Lol p I-specific mAb FMC Al and IgE binding to an allergic human serum pool...

... in cherry. Methods: Characterization was performed using IgE immunoblotting and immunoblot inhibition, N-terminal sequencing, mass spectroscopy analysis and PCR-based cDNA cloning. Results: A 23-kD protein was identified as IgE-binding component. As all cherry-extract-reactive sera displayed IgE-binding...

... patient reacted with C hordeins, various ω gliadins, and γ-75 secalin, but very weakly with γ-35 secalin and LMW-GS. Recombinant γ-3 hordein inhibited strongly but not totally the WDEIA patient’s IgE binding to prolamins. No such inhibition could be observed for the HHWP patient’s IgE. Conclusions...

... and purify the recombinant proteins and to compare their IgE-binding capacities to those of the natural proteins. Methods: cDNA clones coding for profilin were obtained by RT-PCR from total RNA of tomato and bell pepper fruits, sequenced and expressed as non-fusion proteins in Escherichia coli...

...P. Poltronieri; M.S. Cappello; N. Dohmae; A. Conti; D. Fortunato; E.A. Pastorello; C. Ortolani; G. Zacheo Background: Almond proteins can cause severe anaphylactic reactions in susceptible individuals. The aim of this study was the identification of IgE-binding proteins in almonds...

...Ratna Gupta; Bhanu P. Singh; Susheela Sridhara; Shailendra N. Gaur; Raj Kumar; Vijay K. Chaudhary; Naveen Arora Background:Curvularia lunata is an important inhalant allergen. The present study was undertaken to investigate the shared IgG- and IgE-binding components among seven Curvularia species...

... major IgE-binding proteins of 32 and 34 kD from banana were identified as class I chitinases with a hevein-like domain further explaining the cross-sensitization between Hevea latex and fruits [ 37 ]. Among the PR-4 proteins, class II chitinases have been identified from tobacco and tomato...

... of a birch pollen allergen, Bet v III, representing a novel class of Ca 2+ binding proteins: Specific expression in mature pollen and dependence of patients’ IgE binding on protein–bound Ca 2+ . EMBO J 1994; 13:3481–3486. 5. Suphioglu C, Ferreira F, Knox B: Molecular cloning and immunological...

...) with specificity for the major birch pollen allergen Bet v 1 were established from a birch–pollen–allergic patient who had received birch– pollen–specific immunotherapy. The influence of the Bet v 1–specific IgG antibodies on IgE binding to Bet v 1 was investigated. BAB2 was expressed in Escherichia coli...

... a a Laboratory of Toxicology, Institute of Pharmacological Sciences and b 5th Pediatric Department, University of Milan, Italy Key Words IgE binding Structure modifications Allergy Epitopes Immunochemistry Effects of Structure Modifications on IgE Binding Properties of Serum Albumins Abstract Background: Bovine...