... as an example to establish a novel quality control strategy, which was based on chromatography to isolate the allergen, and on enzyme-linked immunosorbent assay to verify the IgE reactivity of the isolated allergen. Methods: The nucleotide sequence encoding Der f 2 was codon-optimized and cloned into pET-28a...

... with an unidentified genetic background). ASDIS was induced by the simultaneous intravenous injection of anti-ovalbumin IgE and fluorescein isothiocyanate (FITC)-ovalbumin, along with Evans blue – a recognized vascular permeability indicator. Anaphylaxis and scratching behavior were monitored through rectal...

... were included in this study; urticaria activity score (UAS7) was recorded and the treatment response was judged based on it. Levels of total serum IgE, anti-high-affinity IgE receptor (FcεRI) IgG, and basophils CD63 and FcεRI expressions, and D-dimer of all patients were determined and analyzed...

...Jorge Sánchez; Yuliana Toro; José-Fernando Cantillo; Dalgys Martínez; Enrique Fernández-Caldas; Ricardo Cardona; Leonardo Puerta Background: Exposure to mosquitoes in the Tropics is perennial, and their somatic and saliva antigens have shown IgE binding capacity, although it is not clear whether...

... of children randomized to the peanut avoidance arm of the LEAP trial, we monitored the development of epitope-specific (ses-)IgE and ses-IgG4 from 4–11 months to 5 years of age. Objective: The aim of the study was to evaluate the prognostic ability of epitope-specific antibodies to predict the result...

... not seem to find a proper positioning in the classic classifications and phenotypes of chronic rhinitis, and its pathophysiology relies specifically on the presence of local IgE. These patients in fact have a suggestive clinical history of allergic rhinitis in the presence of negative skin prick tests...

... patients. Objectives: The aim of this study was to evaluate the diagnostic performance of anti-Der p 10 IgE as a predictor of shrimp allergy. Methods: A nested case-control study was carried out with eighty-six allergic rhinitis patients sensitized to mite ( Dermatophagoides pteronyssinus ) and shrimp...

... can contribute to the clinical manifestation of MCASs. These conditions include hereditary alpha tryptasemia, mastocytosis with an expansion of clonal KIT -mutated mast cells, atopic diathesis, and overt IgE-dependent and IgE-independent allergies. Several of these conditions have overlapping...

... in both pruritus models showed synergism in terms of the antipruritic effect. Our results showed that NO did not play a role in the antipruritic effect of WIN 55,212-2 and 17-AAG. Increased plasma IgE levels with AEW treatment decreased with the administration of 17-AAG (5 mg/kg, i.p.) and WIN 55,212-2...

...Maria Suprun; Scott H. Sicherer; Robert A. Wood; Stacie M. Jones; Donald Y.M. Leung; A. Wesley Burks; David Dunkin; Marc Witmer; Galina Grishina; Robert Getts; Mayte Suárez-Fariñas; Hugh A. Sampson Introduction: Molecular studies of hen’s egg allergens help define allergic phenotypes, with IgE...

... patients. However, the clinical relevance of shrimp sensitization in patients with allergic rhinitis (AR) has been poorly studied. The aim of this study was to determine the prevalence and the clinical relevance shrimp IgE sensitization in AR patients sensitized to Dermatophagoides pteronyssinus. Methods...

...Burcin Beken; Velat Celik; Pınar Gokmirza Ozdemir; Mehtap Yazicioglu Introduction: The skin prick test (SPT) is a reliable method to confirm sensitization in IgE-mediated allergic diseases; however, it has been reported to be affected by several personal and environmental factors. Our objective...

..., and 21 with NAR were included. Patients who met the inclusion criteria were selected to undergo the following procedures at baseline: medical examinations, nasal provocation test (NPT), detection of nasal-specific IgE to birch as well as basophil activation test (BAT). All immunological parameters were...

..., instructions or products referred to in the content or advertisements. Mast cells Allergy Anaphylaxis IgE Mast cell activation Tryptase Mast cells (MCs) are tissue-resident multifunctional effector cells of the immune system [ 1-3 ]. These cells produce not only an array of pro-inflammatory...

...Samuel Roethlisberger; Olfa Karoui; Daria Mapelli; Régine Audran; Vincent Aubert; Laurie D. Girard; Fabien Rebeaud; Annette Leimgruber; Guillaume Buss; Joël Duc; Felicitas Langner-Viviani; Iwan Maerki; François Spertini Background: Clinically meaningful specific IgE determination is an important...

... and potentially fatal allergic disease or hypersensitivity reaction with variable clinical presentation, which usually occurs suddenly after contact with a triggering antigen. IgE-mediated reactions frequently underlie the development of anaphylaxis; however, other pathways have also been described [ 1 ]. Due...

... subcutaneous immunotherapy (SCIT) is similar in patients sensitized to DP alone or to both DP and BT. Method: Ninety-five children (5–17 years old) affected by asthma with rhinitis and sensitized to both DP and BT received 3 years of DP-SCIT. Clinical symptom and medication scores, serum-specific IgE and IgG 4...

...Alireza S. Nejad; Donald W. MacGlashan Jr. Background: The characteristics of the aggregation reaction that follows allergen binding to cell surface IgE on basophils and mast cells depend on a variety of factors that include the density of IgE and the affinity of the allergen for IgE. For simple...

...Sarah Whiteside; Marketa Markova; Alex Chin; Cynthia Lam; Poonam Dharmani-Khan; Monica Modi; Faisal Khan; Jan Storek Background: Atopy is defined as excess allergen-specific IgE (A-IgE). IgE is produced by plasma cells that differentiate from allergen-specific B cells. B cells are known...

..., occur. Allergic reactions to Aedes communis (Ac) may be underestimated due to the lack of reliable diagnostic tools. In this multicenter study, 205 individuals reporting large local reactions to Ac were enrolled and studied for cutaneous or IgE reactivity to Ac, Blattella germanica , Penaeus monodon...