...S.T. Miller; N.P. Barney; D.A. Gamache; J.M. Spellman; J.M. Yanni Background: Mast cells have been identified in the choroid of numerous species including man. However, functional studies involving these human mast cells have not been reported. In the current studies, the secretory response...

... was recently shown to be released from human platelets stimulated with thrombin [Kameyoshi et al: J Exp Med 1992;176:587–592]. Employing postembedding immunocytochemistry, we could detect RANTES and for the first time also MlP-1α within the α-granules of human platelets. To date, MlP-1α was not reported...

... to localize histamine in subcellular sites of mast cells. The DAO-gold method showed that isolated human lung mast cells contained abundant histamine in their cytoplasmic granules, a conclusion which was verified by a large number of specificity controls. We also studied mast-cell-rich eyelid lesions which...

... in the present work that human eosinophils can synthesize interferon (IFN)-γ and IL-10, by RT-PCR, in situ hybridization and immunostaining. Double-labelling procedures revealed the coexpression of IL-5 and IL-10 but not IL-5 and IFN-γ, indicating the existence of subpopulations of eosinophils expressing type 1...

... the numerous pairs of surface adhesion molecules, the CD23/CD21 pair seems to play a key role in the generation of IgE. The CD23 molecule is positively and negatively regulated by factors which increase or decrease IgE production, respectively. Antibodies to CD23 have been shown to inhibit IL-4-induced human...

... to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Human Guinea pig Eosinophil Neutrophil Monocyte Lymphocyte Tumor necrosis factor-α Interleukin-2 Clinical Features, Management and Pharmacology of Allergic Diseases...

...H. Yilmaz; K.L. Morgan; J.M. Roe Cross-reactivity between ovine and human IgE was investigated using monoclonal antibodies to human IgE heavy chain. Western immunoblotting, reverse cutaneous anaphylaxis (RCA), passive cutaneous anaphylaxis inhibition (PCAI) and reverse passive cutaneous anaphylaxis...

.... Chemotaxis Allergy Human Eosinophils Lung Allergic alveolitis Original Paper Int Arch Allergy Immunol 1992;98:233-238 Lena H3leansson* Birgitta Schmekel* - b a Department of Clinical Chemistry, University Hospital, Uppsala; b Department of Human Pharmacology, Astra Draco AB, Lund, Sweden Increased...

...Kiyoko Tanaka; Masayoshi Abe; Nobuaki Shigematsu We studied the influence of a selective 5-lipoxygenase inhibitor, AA861, on the generation of the superoxide anion (O 2 –– ) and the lipoxygenase metabolites by human polymorphonuclear leukocytes (PMN). PMN produce O 2 –– in a dose-dependent manner...

...Janet L. Macpherson; Angel Lopez; Steven A. Krilis Metachromatic staining cells with mast cell and basophil characteristics were grown from normal human bone marrow, both in co-culture with a human mast cell-like strain (HBM-M) and when maintained in the presence of conditioned medium derived from...

... to determine the effect of different allergen concentrations on human house dust mite (HDM)-specific T cell production of IL-4 and IFN-γ, proliferation and apoptosis. Methods: HDM-allergic donor PBMC were cultured for 14 days with different concentrations of HDM extract (1, 10 and 100 µg/ml). T cell...

...Annika Loftenius; Jan Ekstrand; Erna Möller Mercuric chloride (HgCl 2 ) has been proposed to be a mitogen for human blood lymphocytes in vitro. In our previous study, we demonstrated that HgCl 2 preferentially stimulates the CD4+ T cell subset to blast transformation and DNA synthesis...

... was also significantly reduced, not only in the case of human eosinophils but also in a rat eosinophil-mast cell model of cytotoxicity. Taken together, these results indicate that lodoxamide can exert potent inhibitory effects on eosinophil activation in vitro combined with a strong inhibition...