... + CD56 + cells were measured by flow cytometry in 52 confirmed hospitalized patients with COVID-19 at the day of admission and after 7 days of care. Clinical response was defined as improvement in symptoms (fever, dyspnea, and cough as well as blood oxygen saturation), and patients who met these criteria...

.... Siglec-8 is a potential therapeutic target in eosinophilic asthma. Objectives: The aim of this study was to determine Siglec-8 levels on eosinophils and basophils recruited during lung inflammation. Method: We analyzed surface Siglec-8 by flow cytometry on cells obtained by bronchoalveolar lavage (BAL...

... tested before VIT (T0), at 1 week (T1w), at 10 weeks (T10w) and at 21 weeks (T21w), and, for a subgroup of 17 patients, at 3-5 years (T3-5y). Basophil activation (expressed in % CD63 and in the area under the curve) and BF activity were measured by flow cytometry using the CCR3/CD63 protocol. Results...

... possible, for ethical and practical reasons. Therefore, the validation of new cellular tests such as basophil activation testing (BAT) was necessary. This review focuses on the applications of BAT in IDHRs. Methods: A literature search was conducted, using the words basophil, flow cytometry, immediate drug...

... against these allergens were determined before and after 4 or 5 months of maintenance SCIT. Basophil activation was assessed by monitoring CD203c upregulation on flow cytometry. Results: JCP-based SCIT significantly reduced the basophil activation caused by tomato fruit (p = 0.03) and JCP (p < 0.001...

... children were enrolled to the study. Peripheral blood mononuclear cells were stained with monoclonal antibodies (anti-CD25-CD4-CD127-FoxP3-CD69-CD71) and evaluated using flow cytometry. Tregs were identified as CD4+CD25 +/high FoxP3+CD127- T cells. Results: The percentage of Tregs in allergic patients (2.3...

... to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Allergen stimulation Children Flow cytometry Oral food challenge Wheat allergy Wheat is the most widely consumed food grain and is one of the six most common...

.... Allergic rhinitis Basophils Flow cytometry Nasal allergen challenge Peak nasal inspiratory flow Allergic rhinitis is a global health problem that affects social life, sleep, school and work performance, regardless of gender, age and social and ethnic background [ 1 ] . Although a state...

...:4872–4877. 4. Sabbah A, Sainte-Laudy J: Flow cytometry applied to the analysis of lymphocyte and basophil activation. ACI Int 1996;8/4:116–120. 5. Sabbah A, Lauret MG, Maillard H: Preliminary study of basophil activation test for drug allergy using anti-membrane antibodies and flow cytometry...

...M.L. Sanz; A.L. de Weck; C. Uasuf; P.M. Gamboa; M. Chazot The in vitro diagnosis of drug allergy represents a difficult problem, since up to now there is no technique reliable enough to confirm this diagnosis with certainty. Recently flow cytometry has been used as an in vitro diagnostic...

...Salvatore Chirumbolo A wide range of reported evidence in the literature has shown that the quantification of basophil activation by flow cytometry (basophil activation test, BAT) has proven to be a useful tool for the assessment of immediate-type responses to allergens mediated by IgE or other...

...-associated surface antigens (CD11b/CD63/CD123/CD203c/CD294) in characterizing and monitoring subjects with nut allergy. Methods: We used flow cytometry to analyze basophils at baseline (without any activation) and after ex vivo stimulation of whole blood by addition of nut or other allergens for 2, 10...

... of surface markers of NK cells were performed on freshly isolated peripheral blood mononuclear cells using flow cytometry. Results: Patients with AR had a higher percentage of NK cells compared to nonatopic subjects. The mean percentage of IL-4+ NK cells was significantly higher and that of IFN-γ+ NK cells...

... of blocking antibodies. In addition, the number of CD4 + CD25 + T cells and their expression of chemokine receptors were investigated by flow cytometry. Results: Phl p 1 and Phl p 5 induced IL-10 production, which down-regulated proliferation and cytokine production, in PBMC cultures from atopic but not from...

... and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. CD4+ T cells Epidermal cells Flow cytometry Langerhans cells Mice Popliteal lymph node assay Platinum compounds...

... with 7 monoclonal antibodies to epitopes on the variable region of the α- and β-chain by flow cytometry. Cell lines of 9 patients were established with Chi t 1–9, 6 with tetanus toxoid and 6 with phytohaemagglutinin in the presence of interleukin 2. In addition, non-stimulated lymphocytes as well...

...* Christine Lautenschlägerc Jürgen Languora Institute o f Medical Immunology and Clinic o f I lean and Thoracic Surgery. Martin Luther University Halle and Institute o f Medical Epidemiology and Biometry and Medical Informatics, Halle, Germany Key Words Flow cytometry Immunophénotype Non-small-cell lung...

.... Eosinophil Bronchial asthma Eosinophilic pneumonia CD4 HLADR CD45RO Flow cytometry Int Arch Allergy Immunol I995:108(suppl 1):6 8 Shoichi Sakamoto r Kazuliiko Okih Hisaho Takahashih Yukako Arakawah Hironobu Sugita1 Yosinori Kawabata" Kenichi Takeuchi Nobukazu Tomichi1 The Research Institute...

..., instructions or products referred to in the content or advertisements. Asthma, bronchial CD44 Flow cytometry CD16 VLA-4 ImmAunlloelroggyy.International Archivesof Int Arch Allergy Immunol 1997; 114(suppl 1):67 71 Kunio Sarto11 Kohei Yamauchia Hiroki Hoshi11 Masaaki Houmaa Gen Tamuraa Kunio...

... nickel stimulation in vivo and in vitro. Nickel-induced lymphocyte cultures were used in vitro, the cells being examined with monoclonal antibodies (Mabs) and by flow cytometry. Mononuclear cells from skin biopsies of in vivo cutaneous nickel reactions were studied with Mabs and immunohistochemistry...