... activation assays. T-cell proliferation experiments demonstrated that B5M preserved the main T cell epitopes of Bos d 5. Immunization of rabbits with B5M induced protective IgG antibodies that blocked the binding of patients’ IgE antibodies to the wild-type allergen and inhibited the degranulation...

...Lisbeth Ramírez Caballero; Christoph Kny; Regina Treudler; Jan C. Simon; Karolin Kern; Uta Jappe; Michael Szardenings Background: In pollinosis patients, allergen-specific antibody titers show seasonal variations. Little is known about these variations at the epitope level. Objectives: We aimed...

...Kerrie Vaughan; Bjoern Peters; Mark Larche; Anna Pomes; David Broide; Alessandro Sette The recognition of specific epitopes on allergens by antibodies and T cells is a key element in allergic processes. Analysis of epitope data may be of interest for basic immunopathology or for potential...

...Birgit Simon-Nobbe; Rimantas Kodzius; Andrey Kajava; Fátima Ferreira; Andreas Kungl; Gernot Achatz; Reto Crameri; Christof Ebner; Michael Breitenbach We present here the mapping and detailed investigation of an IgE-binding structural epitope of C. herbarum enolase. Additionally we are showing...

... an extremely high allergenic activity in skin test experiments which in some cases equaled that of the complete Phl p 5A allergen. The rPhl p 5A domain thus represents an allergen fragment containing several IgE epitopes in a configuration optimal for efficient effector cell activation. We suggest the rPhl p...

... and nonconjugated peptide induced both IgG and IgE antibodies in Balb/c mice that recognized both native Alt a I allergen and peptide-BSA conjugate. Since the free peptide was able to induce antibodies in vivo, the peptide may also possess a T cell epitope. 14 11 1994 13 06 1995 4 9 2009 ©...

... Epitope International Arrimes of Allergy , Immunology' II. A lle rg e n s Int Arch Allergy Immunol 1997;113:125-127 Fatima Ferreirab Kora Hirthenlehner " Peter Brizab Heimo Breitenedera Otto Sch ein et Dieter Kraft3 Michael Breitenbachb Christo fE b n e r a Institute of General and Experimental...

... have been solved by multiple isomorphous replacement. Refinements are underway to 2.4 and 2.0 Å, respectively. In addition, the IgE-reactive epitopes of BPP where identified by screening an epitope expression library with the serum IgE of an allergic individual. Results : BPP exhibits an α/β-fold which...

... Key Words Cat Ig E antibody IgG 4 antibody Peptide Epitope Abstract Specificities o f IgE and IgG4 antibodies in 12 cat-allergic patients were com­ pared with respect to their reactivity towards 3 IgE-binding synthetic peptides of Felis domesticus allergen 1(Feld I): peptides 25-38 and 46-59 of chain...

...H. Vik; S.K. Steinvåg; S. Elsayed An N-terminal peptide of the major allergen of birch (Bet v I 23-38) was selected for studying the activity of this segment on the basis of optimal hydrophilicity as it was tentatively suggested to be a surface exposed epitope. In addition two control peptides...

... of the autoimmune T cells undergo apoptosis rather than further proliferation. 3 9 2009 Autoimmunity T lymphocyte Activation Central nervous system Experimental autoimmune encephalomyelitis Epitope Apoptosis Controversy: The Role of the Target Organ in the Development of Autoimmune Disease Int...

... on chemical deglycosylation of the glycoproteins. Enzyme-linked immunosorbent assay inhibition showed that the majority (up to 70%) of the anti- Cry j I rabbit IgG antibodies recognized the oligosaccharide moiety of Cry j I. The carbohydrate epitopes were found to be only partially involved in the binding...

..., and recognized OVA presented by HLA-DR10. Based on the response of the TCL to synthetic OVA peptides, it was found that the TCL recognized OVA 323–339, which is a major T cell epitope presented by murine I-A d . The TCL secreted high levels of IL-5, but undetectable amounts of IL-2, interferon-γ, and IL-4 when...

...Tomoko Kawashima; Madoka Taniai; Mitsuko Usui; Shunsaku Ando; Masashi Kurimoto; Tyoku Matuhasi The 4 anti-Cry jI mAbs showing an epitope specificity different from each other, 046, 029, 026 and 027, were selected to analyze the structure of the antigenic determinant for each mAb on a Cry jI...

... occur as opportunists and about 100 are known to elicit mycoses in man and animals [ 1 ]. More than 80 mold genera have been shown to induce type I allergies in susceptible persons, whereas allergenic proteins have been identified in 23 fungal genera. Allergy Cross-reactivity Epitope Fungi IgE...

...Paula J. Busse; Kirsi-Marjut Järvinen; Leticia Vila; Kirsten Beyer; Hugh A. Sampson Background: Caseins are the major allergens responsible for cow’s milk allergy (CMA). We have previously identified the IgE-binding epitopes of the major cow’s milk (CM) proteins except for α s2 -casein. Methods...

...). Heterogeneous glycosylation of this Asn residue could be a source of the multiplicity of natural Hev b 2. Possible participation of the carbohydrate epitopes of latex β-1,3-glucanase isoenzymes in their IgE-binding capacity and cross-reactivity was investigated in this study. Methods: β-1,3-Glucanase isoenzymes...

..., this form of therapy is associated with the risk of systemic anaphylaxis, which, when severe, can be life threatening. A potentially significant reduction in the incidence of IgE-mediated events during immunotherapy may be achieved by the use of short peptides corresponding to T cell epitopes which...

... and their three–dimensional structures are similar. Lipocalins share common biological functions, predominantly related to the transport of small hydrophobic molecules, such as vitamins and pheromones. Immune reactivity to lipocalin allergens is not well known. In Bos d 5, the IgE–binding epitopes are spread...

... cell epitopes of BLG by the Pin–ELISA method. Furthermore, we wanted to investigate a possible association of the IgE recognition patterns in sera of patients with BLG sensitization and the type of clinical reactions following contact with cow’s milk. Methods: Overlapping sequential decapeptides...