... concentrations of total IgE, IL-5, eotaxin, ECP and sCD23 (table  1 ). In accordance with this, the number of eosinophils was significantly higher in NP samples compared to controls. Skin prick tests were positive in 6 of 15 controls and 9 of 16 NP patients. Within the NP group, no impact of atopy was found...

... inflammation is still obscure. The purpose of the present study is to clarify the role of Gal-9 in human eosinophilic pulmonary inflammation in comparison with eotaxin (CCL11). Methods: We measured the levels of Gal-9 and eotaxin in the bronchoalveolar lavage fluid (BALF) of patients with acute and chronic...

.... Although eosinophils are thought to play a crucial role in corneal tissue damage in severe ocular allergy, the mechanisms of eosinophil recruitment to the cornea has not been fully clarified. Eotaxin has been found in tears of severe allergic patients with corneal ulcer. In this study, we investigated...

... to the pathogenesis of asthma including airway inflammation and remodeling. We focused our study on the regulation of chemokine expression by cytokines and analyzed the mechanisms of eotaxin/CCL-11 expression in ASM cells. Methods: Human ASM cells were cultured in vitroand treated with IL-4, interferon-γ (IFNγ...

...D. Schaefer; J.E. Meyer; R. Pods; W. Pethe; J. Hedderich; C. Schmidt; S. Maune Background: Nasal polyposis is mostly associated with eosinophilia of mucosal tissue. This points to the implication of CC chemokines in nasal eosinophilia. Recently the CC chemokine eotaxin-2 (CCL24) was identified...

...: Blood eosinophils were purified using Percoll and anti-CD16 antibody-coated magnetic beads. Expression of PDGFR mRNA was examined by RT-PCR. After stimulating eosinophils with eotaxin, the phosphorylation of MAP kinases was examined by Western blotting with the antiphosphospecific MAP kinase antibody...

...Satoshi Matsukura; Fumio Kokubu; Masatsugu Kurokawa; Mio Kawaguchi; Hideki Kuga; Koushi Ieki; Miho Odaka; Shintaro Suzuki; Shin Watanabe; Hiroko Takeuchi; Robert P. Schleimer; Ulrike Schindler; Mitsuru Adachi Background: Glucocorticoids are known to repress the expression of CC chemokine eotaxin...

...Sung Woo Park; Do Jin Kim; Hun Soo Chang; Sang Jun Park; Young Mok Lee; Jai Soung Park; Il Yup Chung; June Hyuk Lee; Choon-Sik Park Background: Airway eosinophilia is frequently observed during acute exacerbation of asthma. Interleukin-5 (IL-5) and eotaxin are directly involved in the airway...

...-4, IL-5, IL-10 and eotaxin were detected in bronchoalveolar lavage fluid of both WT and IL-5RKO mice following exposure to Asc. Airway eosinophilia and AHR were seen only in WT mice, but not in IL-5RKO mice. We conclude that IL-5 appears to be required for the accumulation of eosinophils and AHR...

... and stained with propidium iodide to detect apoptotic ones. Results: We found that the spontaneous survival of eosinophils was reduced by treatment of the cells with anti-CC chemokine receptor-3 (CCR3) antibody (Ab). Moreover, eotaxin-1, eotaxin-2, and eotaxin-3 all prolonged the survival of eosinophils...

...Ken Fukuda; Naoyuki Yamada; Youichiro Fujitsu; Naoki Kumagai; Teruo Nishida Background: The chemokine eotaxin is a potent and selective chemoattractant for eosinophils. The production of eotaxin by corneal fibroblasts likely contributes to eosinophil infiltration into the corneal stroma...

... than nonasthmatic control subjects. Eotaxin, a potent eosinophil-specific chemotactic factor, is increased in the lower respiratory tract of allergic patients. Recently, lung fibroblasts have been reported to produce eotaxin and are suggested to be the major cellular source of eotaxin. We postulated...

... in MPO, ECP, TRY, eotaxin, interleukin (IL) 4 and IL-5 concentrations to evaluate the role of neutrophils, eosinophils and mast cells in the induction of skin lesions. Sera from 20 healthy subjects were tested as controls. Methods: Eotaxin, IL-4 and IL-5 serum contents were determined by a solid-phase...

...A. Tomkinson; C. Duez; G. Cieslewicz; E.W. Gelfand Background: The accumulation of eosinophils in the lung is a hallmark of asthma. In addition to cytokines such as IL-5 which are essential, chemokines have been implicated in the recruitment of eosinophils to the airway. In particular, eotaxin has...

...Chang-hao Cui; Kohei Honda; Norihiro Saito; Yoshiyuki Yamada; Satoshi Sannohe; Shigeharu Ueki; Kazuyuki Hamada; Kazutoshi Yamaguchi; Yoshimi Kobayashi; Tetsuya Adachi; Hiroyuki Kayaba; Junichi Chihara Background: The CC chemokine eotaxin not only attracts eosinophils to inflamed sites but also...

... CC chemokine receptor 3 (CCR3) and, to a lesser extent, CCR1. CCR3 is mainly responsible for migration of resting eosinophils, and its specific ligand, eotaxin, represents the most potent chemoattractant for eosinophils. Some reports also suggest the expression of CXC chemokine receptor 1 (CXCR1...

..., regulated upon activation normal T cell expressed and secreted (RANTES) and eotaxins I and II are of particular relevance for the recruitment and activation of eosinophils. Th1 reactions depend upon interferon γ-induced CXC chemokines interferon- inducible protein (IP)-10, interferon-inducible T cell-α...

... infection are not well understood. Eotaxin is a CC chemokine which is a potent and specific agonist for CC chemokine receptor 3 (CCR3). CCR3 is expressed on eosinophils, basophils and T lymphocytes. These cells are known to be key cells in the pathogenesis of asthma. Although it has recently been...

...Hiroshi Saito; Hisako Shimizu; Kazuo Akiyama Normal human bronchial epithelial cells (NHBECs) spontaneously synthesize and store chemokines including eotaxin, RANTES, or MCP-3. We also observed the constitutive expression of mRNA specific for CC chemokine receptor-3 (CCR3) in these cells...

...Misato Miyamasu; Yoshikata Misaki; Masao Yamaguchi; Kazuhiko Yamamoto; Yutaka Morita; Kouji Matsushima; Toshiharu Nakajima; Koichi Hirai Background: Accumulating evidence indicates that eotaxin is the primary mediator of tissue eosinophilia. In the present study, we analyzed the mechanisms...