...Taizo Wada; Yusuke Matsuda; Tomoko Toma; Eiko Koizumi; Hiroyuki Okamoto; Akihiro Yachie Background: Food protein-induced enterocolitis syndrome (FPIES) is an uncommon, non-IgE-mediated food allergy. We recently described a significant increase in fecal eosinophil-derived neurotoxin (EDN) after...

..., instructions or products referred to in the content or advertisements. Respiratory syncytial virus Eosinophil-derived neurotoxin Asthma Respiratory virus Wheezing Eosinophilia There is a dire need to understand the underlying pathophysiology of asthma, including the complex genetic...

... and IgE). Eosinophils from healthy donors were incubated in the wells coated with dansyl-specific immunoglobulins with or without DNS-BSA. Eosinophil activation was monitored by superoxide production and eosinophil-derived neurotoxin (EDN) release. Results: Superoxide production and EDN release...

... to signaling from ICAM-1 and its ligands. The concentrations of eosinophil cationic protein and eosinophil-derived neurotoxin in supernatants of eosinophils were significantly greater (p < 0.05) in the presence of recombinant soluble ICAM-1 than without it. These results suggest that signaling from ICAM-1...

...Hirokazu Nakajima; Gerald J. Gleich; Takeshi Fukuda; Sohei Makino; Hirohito Kita To study the regulation of IL-8 production and release of eosinophil-derived neurotoxin (EDN) by normal blood eosinophils, we isolated eosinophils from healthy individuals and stimulated them with immobilized secretory...

..., 1.44 µm). Apoptosis was determined by annexin V-binding. For the phagocytosis assay, eosinophils were co-cultured with monocyte-derived human macrophages or PMA-pretreated U-937 cells. Phagocytosis was determined by light microscopy and by the eosinophil-derived neurotoxin (EDN) concentration...

..., we investigated the degranulation of eosinophils and mast cells within the normal course of pregnancy and after delivery by measuring the urinary concentrations of eosinophil-derived neurotoxin (EDN) and N-methylhistamine. Methods: Spot urine samples from 65 pregnant women and 15 nonpregnant, age...

... to CysLTs was measured using a 96-well multiwell Boyden chamber. Eosinophils were treated with LTD 4 at 10 –6 M for 60 min followed by incubation for 4 h at 37°C in the presence or absence of IL-5 and eosinophil-derived neurotoxin (EDN) release was evaluated. Results: The expression of the mRNA and protein...

..., and IL-5 in Iscove’s MEM, and proliferated until the 2nd week, when the cell number reached a plateau. Under these conditions, more than 90% of the cells differentiate into mature eosinophils in 3 weeks. The expression of major basic protein and eosinophil-derived neurotoxin in the treated cells...

...Junichiro Morioka; Motohiro Kurosawa; Hiroaki Inamura; Rieko Nakagami; Yutaka Mizushima; Junichi Chihara; Tatsuki Yokoseki; Sinobu Kitamura; Yoshiaki Omura; Masao Shibata Background: Eosinophil-derived neurotoxin (EDN), also called eosinophil protein X (EPX), has been suggested to be a useful...

... or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Eosinophil–derived neurotoxin...