... to distortion of the cellular structures and cellular activities and thus culminate in disease processes. Although the effects of Der p 1 allergen on epithelial barrier integrity and inflammatory response are well-established, its contribution to protease inhibitor production is highly limited. Objective...

... the commercially available major allergens (Der p 1 and Der p 2) in combination with Der p 10 do not detect house dust mite (HDM) sensitization in a number of cases when used alone. The objective was to evaluate the IgE reactivity profiles of these patients using an experimental immunoassay biochip. Methods: Sera...

... on their development. The present study focuses on the influence of nitrogen dioxide (NO 2 ) and the major allergen of the house dust mite Der p 1 on human nasal epithelial cells of nonallergic patients in vitro. Nasal epithelial mucosa samples of 11 donors were harvested during nasal air passage surgery and cultured...

...Hui-Ying Wang; Zhong-Shan Gao; Xiang Zhou; Yu Dai; Wo Yao; Xiao-Feng Zhang; Zhao-Wei Yang; Shan-Dong Wu; Chao-Hui Yu; Xu-Yan Yang; Ronald van Ree Background: The role of specific IgE (sIgE) against Der p 1 and Der p 2 in Chinese patients with house dust mite (HDM) allergy has not yet been well...

...Richard M. O’Brien; Wayne R. Thomas CD8 T cells CD8 T cell epitopes House dust mite Der p 1 Anergy Interferon-gamma Specific immunotherapy © 2001 S. Karger AG, Basel 2001 Copyright / Drug Dosage / Disclaimer Copyright: All rights reserved. No part of this publication may...

...; Thierry Batard; Philippe Moingeon Background: Fusion proteins assembling multiple allergens can be engineered by recombinant DNA technologies in order to produce tools for diagnostic and immunotherapeutic purposes. Herein, we developed and characterized chimeras assembling Der p 1 and Der p 2 allergens...

...W.R. Thomas; W. Smith; B.J. Hales; M.D. Carter Background: Sequence polymorphisms have been found in cDNA clones of commercial house dust mites. Here we report on the polymorphisms of Der p 1 found in environmental mites and the effect of the polymorphisms on T cell proliferation. Methods...

... from atopic and non-atopic individuals in both the resting state and after stimulation with the major house dust mite allergen Der p 1. Results: Our data show that unstimulated DCs from atopic and non-atopic individuals are very similar at the whole cell proteome level, showing few differentially...

... examined the effect of transgenic (Tg) rice seeds expressing an immunodominant fragment of the group 1 antigen of Dermatophagoides pteronyssinus (Der p 1) on a murine model of asthma. Methods: Mice were orally vaccinated with the Tg or non-Tg rice seeds for 2 weeks, then they were immunized...

... as to the route of exposure by which sensitization occurs. Cystatin A is a skin-derived dominant inhibitor against proteolytic activity of major mite allergens, Der f 1 and Der p 1, and blocks the upregulation of IL-8 release from human keratinocytes stimulated with the allergens. We analyzed whether...

...Enrique Fernández-Caldas; Mayte Gallego; Jerónimo Carnés; Victor Iraola Background: Mite extracts contain potent enzymes. These enzymes, especially Der p 1, may affect the bronchial homeostasis and the amplification of the allergic response. The objectives of this study were to determine how...

... enhances signal intensity. Very recently, buffer solutions designed to enhance signals in immunoassays have been developed and made commercially available. Methods: Sera from mice immunized either with a recombinant form of one of the major mite allergens Der p 1, Der f 1 and Der f 2, or with ovalbumin...

... was analyzed in DNA isolated from peripheral blood mononuclear cells to identify C/C, C/T and T/T genotypes. Serum tests were done for soluble CD14 (sCD14) and dust mite-specific antibody (Der p 1-IgG1). Results: Serum sCD14 levels were not associated with clinical phenotypes (asthma, bronchial hyperreactivity...

...Toshiro Takai; Takeshi Kato; Mikiko Ota; Hiroshi Yasueda; Takatoshi Kuhara; Ko Okumura; Hideoki Ogawa Background: The major house dust mite group 1 allergens Der p 1 and Der f 1 are the most potent indoor allergens. Der p 1 cleaves human cell surface molecules, the low-affinity IgE receptor (CD23...

...Marc Massaer; Pasqualina Mazzu; Michèle Haumont; Mauro Magi; Véronique Daminet; Alex Bollen; Alain Jacquet Background: The major house dust mite allergen Der p 1 is associated with allergic diseases such as asthma. Production of recombinant Der p 1 was previously attempted, but with limited success...

... were the most prevalent house dust mites in Brazil, while D. farinae was rarely found. The aim of this study was to measure Der f 1 and Der p 1 allergen levels in Brazilian asthmatics’ and controls’ homes. Methods: Sixty-four houses (32 asthmatic, 32 control) were visited for dust sampling from five...

... and histopathologic events in airway inflammation produced by intranasal challenge of mice sensitised to the major house dust mite allergen Der p 1. Methods: C57BL/6 mice were immunised subcutaneously with Der p 1 in alum. Mice were bled and challenged intranasally with Der p 1 on day 14 and killed on day 17. Lungs...

... of the immunodominant peptide of Der p 1 (p 1, 110–131) were restimulated with peptide in vitro. Therefore, the aim of this study was to determine if cognate interactions between T cells mediated by Notch/Delta signaling induce and maintain peripheral T cell tolerance. Methods: Using in situ hybridization and viral...

...(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Allergy House dust mite Der p 1 Asthma Murine models Inflammation Clinical models of human allergic diseases...

... to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. T cell Cytokine Allergen Der p 1 Der p 7 Dermatophagoides pteronyssinus Dermatophagoides farinae The importance of the allergens has been based on IgE...