... was to search for potential novel adjuvants for peanut OIT by assessing the capacity of free purified mannan and different toll-like receptor ligands (TLR-Ls) to immunomodulate the responses of human monocyte-derived dendritic cells (hmoDCs) to peanut allergens. Methods: Monocytes were isolated from PBMCs...

... targeting and continuous and on-demand release of allergen. Objectives: The aim of this study is to investigate the effects of a dendritic cells (DCs)-targeting nano-vaccine for AIT. Methods: The nano-vaccine was produced by coupling polylactic-co-glycolic acid (PLGA)-encapsulated ovalbumin (OVA...

... cells 1/2 (Th1/Th2), Th17/regulatory T cells (Tregs), etc. H. pylori can also target dendritic cells to promote immune tolerance and enhance the protective effect on allergic asthma, and this effect relies on highly suppressed Tregs. The remote regulation of lung immune function by H. pylori...

... the acute allergic skin response and shock symptoms were measured. After 18 h, terminal blood samples, mesenteric lymph nodes, and spleens were collected. Whey-specific immunoglobulin (Ig) E and IgG1 levels were measured by means of ELISA. T cell subsets and dendritic cells (DCs) were studied using flow...

... the development of allergic rhinitis, and dendritic cells are also involved in allergic rhinitis. Methods: We explored the consequences of HIF1α deficiency in dendritic cells on allergic rhinitis. Allergic rhinitis in mice was induced by ovalbumin (OVA). The levels of IgE, leukotriene C4 (LTC4), eosinophil...

... homolog 2 (EZH2) in the activation of dendritic cells (DCs) in AIT. Method: In this study, EZH2 expression in circulating myeloid DCs (mDCs) and plasmacytoid DCs (pDCs) were evaluated using flow cytometry. Clinical information from 56 AR patients receiving AIT was collected, including 30 subjects...

... helper 2 (Th2) response plays a crucial role in the process of asthma disease. We have investigated the reaction of cigarette smoke extract (CSE) on Th polarization which is controlled by dendritic cells (DCs). Stimulated by CSE, immature DCs from murine bone marrow showed upregulated levels of TIM4...

...Motohiko Suzuki; Makoto Yokota; Tamami Matsumoto; Shinya Ozaki Background: We previously reported that siRNA-induced CD40-silenced dendritic cells (DCs) inhibit allergic responses and symptoms. However, more potent therapies are needed. To our knowledge, synergic effects of gene silencing in DCs...

... the role of roasted peanut allergens in the initial phase of allergy, where dendritic cells (DCs) play a key role. We sought to analyze differences in the internalization of Ara h 3 from raw and roasted peanut by immature monocyte-derived DCs (MDDCs) and the implication of the mannose receptor...

... against an epitope of the extracellular domain of TLR2, was identified. The effect of the antibody on dendritic cells was assessed by immunohistochemistry, Western blot, and flow cytometric analysis. The effect of TSP-2 in a murine asthma model induced with ovalbumin (OVA) was assessed. The model...

...Leonie Hussaarts; Maria Mardalena Martini Kaisar; Arzu Tugce Guler; Hans Dalebout; Bart Everts; André M. Deelder; Magnus Palmblad; Maria Yazdanbakhsh Background: Dendritic cells (DCs) are the sentinels of the immune system. Upon recognition of a pathogen, they mature and migrate to draining lymph...

.... Tolerance Airway epithelium Dendritic cells T cells Immune homeostasis Co-culture models All cavities and surfaces throughout the body contain a layer of epithelial cells and are thus often the first cells to interact with external antigens. The skin is the most straightforward example...

... have the capacity to enter into specific organs or organ systems [ 1 ]. These features are indispensable for the activation of other cell types, such as lymphocytes, macrophages and dendritic cells. Consequently, the role of CD4 + T cells is largely indirect, which gave them the name ‘helper' cells...

...Ryosuke Murakami; Yohko Nakagawa; Masumi Shimizu; Ayako Wakabayashi; Yasuyuki Negishi; Takachika Hiroi; Kimihiro Okubo; Hidemi Takahashi Background: Two major distinct subsets of dendritic cells (DCs) are arranged to regulate immune responses: DEC-205+ DCs drive Th1 polarization and 33D1+ DCs...

...Azad Saei; Jamshid Hadjati Dendritic cells (DCs) as professional antigen-presenting cells are able to induce immunity and tolerance in different conditions, which leads to strong immune responses against hostile agents or tolerance to self-antigens. Although for subsets of DCs, a regulatory...

... range of diseases and is mediated by expression of the ST2 receptor in many immune cells. As the effects of IL-33 on dendritic cells (DCs) remain controversial, we investigated the ability of IL-33 to modulate the functions of these cells. Methods: DCs were derived from mouse bone marrow...

...-derived dendritic cells (mDCs) expressing Toll-like receptor (TLR)2 and TLR4 in the peripheral blood of 47 EGPA patients and 40 bronchial asthma patients (who did not have EGPA) and calculated the percentages of monocytes and mDCs that produced IL-23p19 and IL-27 in response to lipopolysaccharide (LPS...

... of allergic asthma, more severe airway inflammation in female mice is associated with higher levels of T helper type 2 (Th2) cytokines. The aim of this study was to investigate the contributions of CD4 + T cells and dendritic cells (DCs) to the differences in Th2 cytokine production between sexes. Methods...

... . In addition, Alternaria produces potent Th2-like adjuvant effects in the airway. Therefore, we hypothesized that Alternaria may inhibit Th1-type defense mechanisms against virus infection. Methods: Dendritic cells (DCs) were generated from mouse bone marrow. The functional responses were assessed...

...Chin-Lai Lee; Shih-Hsien Hsu; Yuh-Jyh Jong; Chih-Hsing Hung; Jau-Ling Suen Background: Histamine exerts diverse effects on immune regulation through four types of histamine receptors (HRs). Among them, type 1 receptor (H1R) plays an important role in allergic inflammation. Dendritic cells (DCs...