...Shan-Shan Li; Xue-Li Pu; Jing-Ru Zhou; Chun-Sheng Wang; Jia-Ling Wang; Xilizati Kulaixi; Jian-Rong Ye Introduction: This study aimed to investigate the role of Raf kinase inhibitor protein (RKIP) in degranulation induced by echinococcal cyst fluid (EgCF) in bone marrow-derived mast cells (BMMCs...

... Graves’ disease and induced thyrotoxicosis, we detected a significant increase in the levels of interferon-γ, active interfollicular infiltration and degranulation of mast cells, and the intrathyroid overexpression of CD86. Complex analysis of the rat’s thyroid morphofunctional state and systemic...

... concentrations of anti-FcεRI α-chain mAb (CRA-1 mAb), and their cell functions were assessed. Results: Basophils preincubated with CRA-1 mAb at as low as 1 ng/ml for 1 h showed significantly enhanced degranulation in response to various secretagogues such as MCP-1, FMLP, leukotriene B 4 and Ca ionophore A23187...

... immunoglobulin E-mediated degranulation in mast cells by phosphorylating SNAP-23, the target-membrane soluble N-ethylmaleimide-sensitive fusion factor attachment protein receptor (SNARE). In addition to IKK2, a recent study has shown that ELKS, a regulatory subunit of the IKK complex, also induces...

... activation, particularly the degranulation response and LTC 4 secretion after prolonged culture of mast cells with high-glucose medium. Moreover, it is suggested that increased phosphorylation of cPLA 2 at the Ser505 residue contributes to the enhancement of LTC 4 secretion. 4 6 2010 © 2010 S...

..., instructions or products referred to in the content or advertisements. Protein SV-IV Mast cells Basophils FcεRI receptor Histamine Degranulation Blastocyst implantation Seminal vesicle protein number 4 (SV-IV, according to its mobility in SDS-PAGE; precursor UniProtKB/Swiss-Prot ID...

..., and certain eye diseases. The effector functions of eosinophils appear to be derived primarily from release of lipid mediators and proteins, including cytokines and granule proteins. Eosinophil degranulation results in the release of several cytotoxic cationic granule proteins. Furthermore, release...

... advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Mast cell Xid mice Secretion Degranulation...

... factor and nerve growth factor affected only LTC 4 production. The optimal preincubation period for priming of LTC 4 production by IL-3 or IL-5 was 90 min, while that for eosinophil cationic protein release was 10 min. These results indicate that degranulation and the generation of lipid mediators...

... and its ligands might induce eosinophil activation and might be involved in degranulation of eosinophil granule proteins, e.g. eosinophil cationic protein and eosinophil-derived neurotoxin. 4 9 2009 © 1995 S. Karger AG, Basel 1995 Copyright / Drug Dosage / Disclaimer Copyright: All rights...

...Takao Fujisawa; Akihiko Terada; Jun Atsuta; Kosei Iguchi; Hitoshi Kamiya; Minoru Sakurai The ability of IL-5 to induce eosinophil degranulation was investigated. Peripheral blood eosinophils from patients with mildly allergic individuals were isolated with CD 16- selection method. Eosinophils were...

...Masahiko Kato; Hirohito Kita; Akihiro Morikawa Degranulation of eosinophils and subsequent release of toxic granule proteins play a key role in allergic diseases such as bronchial asthma. However, little is known about the intracellular signaling mechanism of eosinophil degranulation...

...-hexosaminidase release assays, a leukotriene ELISA, transient transfections with reporter gene constructs of TNFα and MARC, and in addition a TNFα ELISA. Results: Consistent with previously published data in the rat basophilic cell line RBL-2H3, we find that wortmannin as well as DMV prevent the degranulation...

...Shigeru Takafuji; Kenji Tadokoro; Koji lto; Clemens A. Dahinden It is unclear what actually induces eosinophil degranulation in vivo. We examined eosinophil cationic protein (ECP) release from normal human eosinophils (Eos) in response to C3a and C5a. C3a and C5a induced remarkable ECP release when...

... or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Beige rats Chédiak-Higashi syndrome Mast cells Degranulation Granules, giant Original Paper Int Arch Allergy Immunol 1993;100:99-106 Tomoko Jippo-Kanemotoa Tsutomu Kasugaia...

... or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. FcεRI Pyruvate kinase Yeast two–hybrid system Degranulation FcεRI, a tretamer...

... mouse bone marrow-derived mast cells were transferred. Results: Pam3CSK4 and Bifidobacterium pseudocatenulatum JCM 7041 suppressed degranulation of IgE-sensitized mast cells upon antigen stimulation in vitro. Pam3CSK4 also suppressed leukotriene C 4 production triggered by engagement of the high...

... of PI3Ks on the FcεRI-mediated migration toward the antigen (Ag). In the present study, we explored the role of PI3Kγ on mast cell migration. The treatment of bone marrow-derived mast cells (BMMCs) with a PI3Kγ inhibitor, AS605240, significantly repressed FcεRI-induced degranulation and migration...

... or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Hepatocyte growth factor Asthma Eosinophil Reactive oxygen species Degranulation Asthma is a complex chronic airway disorder characterized by allergen-induced airway...

... min at 37°C. After the degranulation (histamine release) reactions were terminated by centrifugation at 4°C for 5 min, the supernatants were collected. Perchloric acid was added to them up to the final concentration of 1.2% to precipitate proteins in the fluid. All samples were kept at 4°C overnight...