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Keywords: Circular dichroism
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Journal Articles
Int Arch Allergy Immunol (2013) 162 (1): 25–38.
Published Online: 27 June 2013
... on effector cells. Our aim was to map the immunodominant regions of shrimp tropomyosin by both IgE-binding and IgE-receptor crosslinking studies. Methods: Five overlapping fragments covering Pandalus borealis tropomyosin were cloned, expressed in Escherichia coli and characterized by circular dichroism...
Journal Articles
Int Arch Allergy Immunol (2013) 160 (3): 221–232.
Published Online: 16 October 2012
... molecular mass and indicated the absence of posttranslational modifications. Circular dichroism spectroscopy revealed virtually identical spectra between recombinant and natural Pan b 1, which together with native PAGE and size exclusion chromatography results indicated a similar structure. Furthermore...
Journal Articles
Int Arch Allergy Immunol (2008) 147 (4): 289–298.
Published Online: 11 July 2008
... on the secondary structure of the protein were recorded by circular dichroism. The level and IgE reactivity of Mal d 2 present in industrially prepared foods were assessed. Results: Purified Mal d 2 consisted of two isoforms. Both harbored carbohydrate moieties and bound serum IgE from apple allergic individuals...
Journal Articles