... pulmonary disease. Despite its crucial biological functions, the mechanisms by which IL-41 mitigates lipopolysaccharide (LPS)-induced acute lung injury (ALI) are not well understood. This study aimed to elucidate the protective effects of IL-41 against LPS-induced inflammation and apoptosis in a murine...

... apoptosis, and Western blotting was used to determine the levels of apoptotic-related proteins (cleaved caspase 3, cleaved polyadenosine-diphosphate-ribose polymerase) and HMGB1, TLR4, and MyD88. Enzyme-linked immunosorbent assay was used to assess the inflammatory response. Hematoxylin-eosin staining...

... unexplored. Methods: Cytotoxicity delivered by various concentrations of LPS and amygdalin on BEAS-2B cells was determined by Cell Counting Kit-8 assay. CVA in vitro models were established via LPS exposure on BEAS-2B cells which underwent amygdalin pretreatment. Cell apoptosis was determined by flow...

... from any ideas, methods, instructions or products referred to in the content or advertisements. Siglec-9 Siglec-E Severe asthma Neutrophils Programmed cell death Apoptosis Intravenous immunoglobulin Asthma is a chronic respiratory disease that affects more than 235 million people...

... and/or lymphocytes). Viability levels were measured using the Alexa Fluor® 488 Annexin V/Dead Cell apoptosis kit (Invitrogen™, Life Technologies, Eugene, OR, USA) according to the manufacturer's instructions. Acquisition was performed using a BD FACSCanto flow cytometer. Viable cells were double-negative...

..., instructions or products referred to in the content or advertisements. Cell death Apoptosis Cross-presentation Apoptotic epitope-specific CD8+ T cells Cell death represents an essential mechanism of cell homeostasis in multicellular organisms in order to regulate morphogenesis and development...

... blood eosinophils, using in vitro systems to test the hypothesis that DHA negatively regulates eosinophil mechanisms through PPARα and PPARγ. Results: Eosinophil apoptosis that spontaneously occurs under normal culture conditions was accelerated in the presence of DHA. In addition, eotaxin-directed...

..., activation-induced apoptosis in peripheral blood. J Allergy Clin Immunol 2000;105:167 (abstr.). T cell Cytokines IgE Eosinophils Apoptosis Atopic dermatitis © 2001 S. Karger AG, Basel 2001 Copyright / Drug Dosage / Disclaimer Copyright: All rights reserved. No part of this publication...

... receptor Antigen-presenting cells Apoptosis NF-kappa B Dendritic cells For decades, studies on FcεRI focused on effector cells of anaphylaxis such as mast cells and basophils. FcεRI on these cells is essential for immediate-type hypersensitivity reactions like allergic rhinitis, urticaria...

...Axel Trautmann; Mübeccel Akdis; Sven Klunker; Kurt Blaser; Cezmi A. Akdis 24 1 2012 Apoptosis is a key event in a variety of physiological and pathological conditions [ 7 ]. Current studies on the role of apoptosis in the pathogenesis of various diseases are focused mainly...

... eosinophils, at the subepithelial region beneath the basement membrane of the airway. Apoptosis is a form of physiological cell death, through which the cellular contents including biologically active substances are kept in the cell membrane and are removed without their harmful effects. So, attempts were...

... of AGEs on various functions and markers of human basophils, including CD11b expression, apoptosis, degranulation, and cytokine production. Results: Flow cytometric analysis indicated that the level of the receptor for AGEs (RAGE) on the surface of freshly isolated basophils was very low but was clearly...

... effect was observed in non-atopic controls except that AZM did inhibit IFN-γ production of their Th0 cells. AZM at a higher dose decreased cell viability by inhibiting CD4+ T cell proliferation and enhanced their apoptosis, an effect similarly observed in Th0 and Th2 cells, and did not differ between...

... MCs and neutrophils were significantly greater in mice with CM than in control mice, and pimecrolimus treatment decreased the numbers of MCs but not neutrophils. Extensive apoptosis of cutaneous MCs was observed in pimecrolimus-treated mice. The cutaneous histamine level was elevated in the mice...

...Kenji Matsumoto; Maki Terakawa; Shuhei Fukuda; Hirohisa Saito Background: Activation of cell surface CD30 by immobilized anti-CD30 monoclonal antibodies (mAbs) induces extremely rapid and intense apoptosis in human eosinophils in vitro. This anti-CD30 mAb-induced eosinophil apoptosis was inhibited...

... eosinophilic pneumonia (AEP and CEP). Furthermore, the biological activities (chemotaxis and apoptosis) of Gal-9 were compared with those of eotaxin using interleukin-5-primed or -unprimed eosinophils. Results: The levels of Gal-9 and eotaxin in the BALF from patients with AEP and those with CEP were higher...

...-10. T-cell inactivation mirrors induction of anergy, T-cell receptor downregulation, and that of CD2, CD4 and CD8 cell surface molecules, as well as of apoptosis. Two waves of apoptosis cripple the T-cell repertoire of superantigen-reactive T cells, an immediate one being induced within 24 h...

...I. Rinner; P. Felsner; D. Hofer; A. Globerson; K. Schauenstein Unlike splenic or blood lymphocytes, rat thymocytes spontaneously undergo continuously increasing apoptosis during culture. In this study we characterized apoptotic thymus cells of rats according to cell size, nuclear dye binding...

...H. Lechner; M. Amort; M.-M. Steger; C. Maczek; B. Grubeck-Loebenstein CD95 (APO-1) is a member of the TNF/nerve growth factor receptor superfamily, which is expressed on the surface of different types of cells. Cross-linking of CD95 leads to the induction of apoptosis. This may be of importance...

... inhibited DNA fragmentation of eosinophils in vitro. TGF-β reversed the effect of IL-5, indicating that TGF-β1 activates the pathway of apoptosis. TGF-β1 significantly suppressed eosinophil protein X release induced by IL-5. These results suggest that TGF-β1 may play a role in the modulation of allergic...