Background: The release of the aeroallergen Bet v 1 from pollen is a major determinant in the etiology of allergic airway disease due to birch pollen. Objective: We determined the release of the major birch pollen allergen Bet v 1 from pollen of birch trees growing in 2 different geographic regions in Germany for 2 consecutive years. Methods: Catkins were collected during pollination in 2002 and 2003 from 82 healthy trees in South (Munich) and West Germany (North Rhine-Westphalia). The release of Bet v 1 from pollen samples was determined by a Bet v 1-specific ELISA. Results: Pollen from South Germany released about 3 times more Bet v 1 than those from West Germany in both 2002 and 2003 (p = 0.034 and p = 0.007, respectively). This was independent of the number of pollen during the pollen flight season. In 2003, the release of Bet v 1 from pollen was more than 5 times higher than in 2002 in both regions (South Germany 6.1 times, p < 0.001; West Germany 5.4 times, p = 0.003). Conclusions: Despite large individual differences, there seem to be regional and year-to-year variations in Bet v 1 release from birch pollen. Therefore, the combination of pollen count and release of Bet v 1 from this pollen must be assessed to estimate Bet v 1 exposure reliably.

Schäfer T, Ring J: Allergien. Dtsch Med Wochenschr 2002;127:781–783.
Krämer U, Link E, Oppermann H, Ranft U, Schäfer T, Thriene B, Behrendt H, Ring J: Studying school beginners in western and eastern Germany: allergy trends and sensitisations 1991–2000. Gesundheitswesen 2002;64:657–663.
Stevens WJ, Ebo DG, Hagendorens MM, Bridts CH, De Clerck LS: Is the prevalence of specific IgE to classical inhalant aeroallergens among patients with respiratory allergy changing? Evidence from two surveys 15 years apart. Acta Clin Belg 2003;58:178–182.
Nowak D, Heinrich J, Jörres R, Wassmer G, Berger J, Beck E, Boczor S, Claussen M, Wichmann HE, Magnussen H: Prevalence of respiratory symptoms, bronchial hyperresponsiveness and atopy among adults: West and East Germany. Eur Respir J 1996;9:2541–2552.
Vieths S, Scheurer S, Ballmer-Weber B: Current understanding of cross-reactivity of food allergens and pollen. Ann NY Acad Sci 2002;964:47–68.
Movérare R, Westritschnig K, Svensson M, Hayek B, Bende M, Pauli G, Sorva R, Haahtela T, Valenta R, Elfman L: Different IgE reactivity profiles in birch pollen-sensitive patients from six European populations revealed by recombinant allergens: an imprint of local sensitization. Int Arch Allergy Immunol 2002;128:325–335.
Markovic-Housley Z, Degano M, Lamba D, von Roepenack-Lahaye E, Clemens S, Susani M, Ferreira F, Scheiner O, Breiteneder H: Crystal structure of a hypoallergenic isoform of the major birch pollen allergen Bet v 1 and its likely biological function as a plant steroid carrier. J Mol Biol 2003;325:123–133.
Chapman MD, Pomés A, Breiteneder H, Ferreira F: Nomenclature and structural biology of allergens. J Allergy Clin Immunol 2007;119:414–420.
Friedl-Hajek R, Radauer C, O’Riordain G, Hoffmann-Sommergruber K, Leberl K, Scheiner O, Breiteneder H: New Bet v 1 isoforms including a naturally occurring truncated form of the protein derived from Austrian birch pollen. Mol Immunol 1999;36:639–645.
Batard T, Didierlaurent A, Chabre H, Mothes N, Bussières L, Bohle B, Couret MN, Ball T, Lemoine P, Focks Tejkl M, Chenal A, Clément G, Dupont F, Valent P, Krauth MT, André C, Valenta R, Moingeon P: Characterization of wild-type recombinant Bet v 1a as a candidate vaccine against birch pollen allergy. Int Arch Allergy Immunol 2005;136:239–249.
Kraft D, Ferreira F, Vrtala S, Breiteneder H, Ebner C, Valenta R, Susani M, Breitenbach M, Scheiner O: The importance of recombinant allergens for diagnosis and therapy of IgE-mediated allergies. Int Arch Allergy Immunol 1999;118:171–176.
Swoboda I, Jilek A, Ferreira F, Engel E, Hoffmann-Sommergruber K, Scheiner O, Kraft D, Breiteneder H, Pittenauer E, Schmid E, et al.: Isoforms of Bet v 1, the major birch pollen allergen, analyzed by liquid chromatography, mass spectrometry, and cDNA cloning. J Biol Chem 1995;270:2607–2613.
Hartl A, Kiesslich J, Weiss R, Bernhaupt A, Mostböck S, Scheiblhofer S, Flöckner H, Sippl M, Ebner C, Ferreira F, Thalhamer J: Isoforms of the major allergen of birch pollen induce different immune responses after genetic immunization. Int Arch Allergy Immunol 1999;120:17–29.
Ferreira F, Hirtenlehner K, Jilek A, Godnik-Cvar J, Breiteneder H, Grimm R, Hoffmann-Sommergruber K, Scheiner O, Kraft D, Breitenbach M, Rheinberger HJ, Ebner C: Dissection of immunoglobulin E and T lymphocyte reactivity of isoforms of the major birch pollen allergen Bet v 1: potential use of hypoallergenic isoforms for immunotherapy. J Exp Med 1996;183:599–609.
Andersson K, Marknell Dewitt A, Mattsson L, Ostling J, Lilja G, Nordlund M, Ballmer-Weber B, Scheurer S, Wangorsch A, Vieths S, Lidholm J: Critical assessment of interchangeability of cross-reactive food allergens in diagnostic tests for specific IgE. 26th Symposium of the Collegium Internationale Allergologicum, Malta, 2006, p 30.
Ghunaim N, Grönlund H, Kronqvist M, Grönneberg R, Söderström L, Ahlstedt S, van Hage-Hamsten M: Antibody profiles and self-reported symptoms to pollen-related food allergens in grass pollen-allergic patients from northern Europe. Allergy 2005;60:185–191.
Neudecker P, Nerkamp J, Eisenmann A, Nourse A, Lauber T, Schweimer K, Lehmann K, Schwarzinger S, Ferreira F, Rösch P: Solution structure, dynamics, and hydrodynamics of the calcium-bound cross-reactive birch pollen allergen Bet v 4 reveal a canonical monomeric two EF-hand assembly with a regulatory function. J Mol Biol 2004;336:1141–1157.
Traidl-Hoffmann C, Kasche A, Jakob T, Huger M, Plötz S, Feussner I, Ring J, Behrendt H: Lipid mediators from pollen act as chemoattractants and activators of polymorphonuclear granulocytes. J Allergy Clin Immunol 2002;109:831–838.
Plötz SG, Traidl-Hoffmann C, Feussner I, Kasche A, Feser A, Ring J, Jakob T, Behrendt H: Chemotaxis and activation of human peripheral blood eosinophils induced by pollen-associated lipid mediators. J Allergy Clin Immunol 2004;113:1152–1160.
Traidl-Hoffmann C, Mariani V, Hochrein H, Karg K, Wagner H, Ring J, Mueller MJ, Jakob T, Behrendt H: Pollen-associated phytoprostanes inhibit dendritic cell interleukin-12 production and augment T helper type 2 cell polarization. J Exp Med 2005;201:627–636.
Jilek A, Swoboda I, Breiteneder H, Fogy I, Ferreira F, Schmid E, Heberle-Bors E, Scheiner O, Rumpold H, Koller HT, Breitenbach M: Biological function, isoforms, and environmental control in the Bet v 1 gene family; in Kraft D, Sehon A (eds): Molecular Biology and Immunology of Allergens. Boca Raton, CRC Press, 1993, pp 39–45.
Swoboda I, Dang TCH, Heberle-Bors E, Vicente O: Expression of Bet v 1, the major birch pollen allergen, during anther development. Protoplasma 1995;187:103–110.
Schäppi GF, Taylor PE, Staff IA, Suphioglu C, Knox RB: Source of Bet v 1 loaded inhalable particles from birch revealed. Sex Plant Reprod 1997;10:315–323.
Namork E, Johansen BV, Løvik M: Detection of allergens adsorbed to ambient air particles collected in four European cities. Toxicol Lett 2006;165:71–78.
Hesselmar B, Åberg B, Eriksson B, Åberg N: Allergic rhinoconjunctivitis, eczema, and sensitization in two areas with differing climates. Pediatr Allergy Immunol 2001;12:208–215.
Kihlström A, Lilja G, Pershagen G, Hedlin G: Exposure to birch pollen in infancy and development of atopic disease in childhood. J Allergy Clin Immunol 2002;110:78–84.
von Berg A, Koletzko S, Grübl A, Filipiak-Pittroff B, Wichmann HE, Bauer CP, Reinhardt D, Berdel D: The effect of hydrolyzed cow’s milk formula for allergy prevention in the first year of life: the German Infant Nutritional Intervention Study, a randomized double-blind trial. J Allergy Clin Immunol 2003;111:533–540.
Hjelmroos M, Schumacher MJ, Van Hage-Hamsten M: Heterogeneity of pollen proteins within individual Betula pendula trees. Int Arch Allergy Immunol 1995;108:368–376.
Schäppi GF, Monn C, Wüthrich B, Wanner HU: Direct determination of allergens in ambient aerosols: methodological aspects. Int Arch Allergy Immunol 1996;110:364–370.
Lorenz RJ: Biometrie. Grundbegriffe der Biometrie. Stuttgart, Gustav Fischer Verlag, 1989.
Pauli G, Oster JP, Deviller P, Heiss S, Bessot JC, Susani M, Ferreira F, Kraft D, Valenta R: Skin testing with recombinant allergens rBet v 1 and birch profilin, rBet v 2: diagnostic value for birch pollen and associated allergies. J Allergy Clin Immunol 1996;97:1100–1109.
Schäppi GF, Suphioglu C, Taylor PE, Knox RB: Concentrations of the major birch tree allergen Bet v 1 in pollen and respirable fine particles in the atmosphere. J Allergy Clin Immunol 1997;100:656–661.
Vrtala S, Grote M, Duchène M, vanRee R, Kraft D, Scheiner O, Valenta R: Properties of tree and grass pollen allergens: reinvestigation of the linkage between solubility and allergenicity. Int Arch Allergy Immunol 1993;102:160–169.
Podoshin L, Gertner R, Fradis M: Treatment of perennial allergic rhinitis with ascorbic acid solution. Ear Nose Throat J 1991;70:54–55.
Ahlholm JU, Helander ML, Savolainen J: Genetic and environmental factors affecting the allergenicity of birch (Betula pubescens ssp. czerepanovii [Orl.] Hämet-Ahti) pollen. Clin Exp Allergy 1998;28:1384–1388.
Tashpulatov AS, Clement P, Akimcheva SA, Belogradova KA, Barinova I, Rakhmawaty FD, Heberle-Bors E, Touraev A: A model system to study the environment-dependent expression of the Bet v 1a gene encoding the major birch pollen allergen. Int Arch Allergy Immunol 2004;134:1–9.
Jaeger S, Berger J: Phenology and human health: allergic disorders. WHO meeting, Rome, 16–17 January, 2003, p 13.
Hallsdóttir M: Birch pollen abundance in Reykjavik, Iceland. Grana 1999;38:368–373.
Helander ML, Savolainen J, Ahlholm J: Effects of air pollution and other environmental factors on birch pollen allergens. Allergy 1997;52:1207–1214.
Weber RW: Meteorologic variables in aerobiology. Immunol Allergy Clin North Am 2003;23:411–422.
Behrendt H, Tomczok J, Sliwa-Tomczok W, Kasche A, Ebner von Eschenbach C, Becker WM, Ring J: Timothy grass (Phleum pratense L.) pollen as allergen carriers and initiators of an allergic response. Int Arch Allergy Immunol 1999;118:414–418.
Estrella N, Menzel A, Krämer U, Behrendt H: Integration of flowering dates in phenology and pollen counts in aerobiology: analysis of their spatial and temporal coherence in Germany (1992–1999). Int J Biometeorol 2006;51:49–59.
Pettenburger K, Tejkl M, Valenta R, Hoffmann-Sommergruber K, Swoboda I, Heberle-Bors E, Breitenbach M, Rumpold H, Kraft D, Scheiner O: Distribution of allergens and allergen-coding mRNAs in various tissues of white birch. Mol Immunol 1992;29:1401–1406.
Spieksma FThM, Emberlin JC, Hjelmroos H, Jäger S, Leuschner RM: Atmospheric birch (Betula) pollen in Europe: trends and fluctuations in annual quantities and the starting dates of the seasons. Grana 1995;34:51–57.
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