Background: Asthma is a life-threatening immediate-type allergic disease. B cell-activating factor (BAFF) is a key regulator of B lymphocyte development and is required to generate and maintain the mature B cell pool. Objectives: To investigate the level of BAFF in the serum of asthma patients and the role of BAFF on T cells. Methods: The BAFF level was measured by enzyme-linked immunosorbent assay. Peripheral blood mononuclear cells (PBMC) from asthma patients were analyzed by flow cytometry. T8.1 cells were used to test the role of BAFF on T cell-antigen-presenting cell (APC) conjugate formation. Results: The BAFF level in patient serum was elevated relative to normal serum. Immunoglobulin E (IgE) concentration and the percentage of CD3+ T and CD19+ B cells vary according to the serum BAFF level. Patients with high BAFF and high IgE (group II) and those with high BAFF and low IgE (group III) show a high ratio of CD3+ T to CD19+ B cells, and the opposite is seen for patients with low BAFF and high IgE (group I) and those with low BAFF and low IgE (group IV). The addition of BAFF increased PBMC proliferation and T cell-APC conjugate formation. BAFF concentration in serum decreased after treatment with antiasthmatic drugs including glucocorticoids and immunosuppressants. Conclusion: These findings suggest that the serum BAFF level is high in both IgE-mediated asthma and non-IgE-mediated asthma and extend our knowledge about the fact that BAFF may play a stimulatory role on the proliferation of T cells. Thus, BAFF could be a parameter to monitor the severity of asthma symptoms.

1.
El Biaze M, Boniface S, Koscher V, Mamessier E, Dupuy P, Milhe F, Ramadour M, Vervloet D, Magnan A: T cell activation, from atopy to asthma: more a paradox than a paradigm. Allergy 2003;58:844–853.
2.
Geha RS, Jabara HH, Brodeur SR: The regulation of immunoglobulin E class-switch recombination. Nat Rev Immunol 2003;3:721–732.
3.
Rackeman FM: A working classification of asthma. Am J Med 1947;33:601–606.
4.
Ostergaard PA: Non-IgE-mediated asthma in children. Acta Paediatr Scand 1985;74:713–719.
5.
Corrigan C: Mechanisms of intrinsic asthma. Curr Opin Allergy Clin Immunol 2004;4:53–56.
6.
Yan M, Brady JR, Chan B, Lee WP, Hsu B, Harless S, Cancro M, Grewal IS, Dixit VM: Identification of a novel receptor for B lymphocyte stimulator that is mutated in a mouse strain with severe B cell deficiency. Curr Biol 2001;11:1547–1552.
7.
Mak TW, Yeh WC: Signaling for survival and apoptosis in the immune system. Arthritis Res 2002;4(suppl 3):S243–S252.
8.
Mackay F, Schneider P, Rennert P, Browning J: BAFF AND APRIL: a tutorial on B cell survival. Annu Rev Immunol 2003;21:231–264.
9.
Mackay F, Browning JL: BAFF: a fundamental survival factor for B cells. Nat Rev Immunol 2002;2:465–475.
10.
Litinskiy MB, Nardelli B, Hilbert DM, He B, Schaffer A, Casali P, Cerutti A: DCs induce CD40-independent immunoglobulin class switching through BLyS and APRIL. Nat Immunol 2002;3:822–829.
11.
Huard B, Arlettaz L, Ambrose C, Kindler V, Mauri D, Roosnek E, Tschopp J, Schneider P, French LE: BAFF production by antigen-presenting cells provides T cell co-stimulation. Int Immunol 2004;16:467–475.
12.
Mackay F, Woodcock SA, Lawton P, Ambrose C, Baetscher M, Schneider P, Tschopp J, Browning JL: Mice transgenic for BAFF develop lymphocytic disorders along with autoimmune manifestations. J Exp Med 1999;190:1697–1710.
13.
Schiemann B, Gommerman JL, Vora K, Cachero TG, Shulga-Morskaya S, Dobles M, Frew E, Scott ML: An essential role for BAFF in the normal development of B cells through a BCMA-independent pathway. Science 2001;293:2111–2114.
14.
Zouali M: B cell diversity and longevity in systemic autoimmunity. Mol Immunol 2002;38:895–901.
15.
Mackay F, Kalled SL: TNF ligands and receptors in autoimmunity: an update. Curr Opin Immunol 2002;14:783–790.
16.
Ware CF: APRIL and BAFF connect autoimmunity and cancer. J Exp Med 2000;192:F35–F38.
17.
Mackay F, Sierro F, Grey ST, Gordon TP: The BAFF/APRIL system: an important player in systemic rheumatic diseases. Curr Dir Autoimmun 2005;8:243–265.
18.
Krumbholz M, Theil D, Derfuss T, Rosenwald A, Schrader F, Monoranu CM, Kalled SL, Hess DM, Serafini B, Aloisi F, Wekerle H, Hohlfeld R, Meinl E: BAFF is produced by astrocytes and up-regulated in multiple sclerosis lesions and primary central nervous system lymphoma. J Exp Med 2005;201:195–200.
19.
Huard B, Schneider P, Mauri D, Tschopp J, French LE: T cell costimulation by the TNF ligand BAFF. J Immunol 2001;167:6225–6231.
20.
Ye Q, Wang L, Wells AD, Tao R, Han R, Davidson A, Scott ML, Hancock WW: BAFF binding to T cell-expressed BAFF-R costimulates T cell proliferation and alloresponses. Eur J Immunol 2004;34:2750.
21.
Wang H, Marsters SA, Baker T, Chan B, Lee WP, Fu L, Tumas D, Yan M, Dixit VM, Ashkenazi A, Grewal IS: TACI-ligand interactions are required for T cell activation and collagen-induced arthritis in mice. Nat Immunol 2001;2:632–637.
22.
Denizot F, Lang R: Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J Immunol Methods 1986;89:271–277.
23.
Michel F, Acuto O: Induction of T cell adhesion by antigen stimulation and modulation by the coreceptor CD4. Cell Immunol 1996;173:165–175.
24.
Ng LG, Sutherland AP, Newton R, Qian F, Cachero TG, Scott ML, Thompson JS, Wheway J, Chtanova T, Groom J, Sutton IJ, Xin C, Tangye SG, Kalled SL, Mackay F, Mackay CR: B cell-activating factor belonging to the TNF family (BAFF)-R is the principal BAFF receptor facilitating BAFF costimulation of circulating T and B cells. J Immunol 2004;173:807–817.
25.
Sutherland APR, Ng LG, Fletcher CA, Shum B, Newton RA, Grey ST, Rolph MS, Mackay F, Mackay CR: BAFF augments certain Th1-associated inflammatory responses. J Immunol 2005;174:5537–5544.
26.
Schneider P, MacKay F, Steiner V, Hofmann K, Bodmer JL, Holler N, Ambrose C, Lawton P, Bixler S, Acha-Orbea H, Valmori D, Romero P, Werner-Favre C, Zubler RH, Browning JL, Tschopp J: BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth. J Exp Med 1999;189:1747–1756.
27.
Hikida M, Johmura S, Hashimoto A, Takezaki M, Kurosaki T: Coupling between B cell receptor and phospholipase C-gamma2 is essential for mature B cell development. J Exp Med 2003;198:581–589.
28.
Claudio E, Brown K, Park S, Wang H, Siebenlist U: BAFF-induced NEMO-independent processing of NF-kappa B2 in maturing B cells. Nat Immunol 2002;3:958–965.
29.
Mecklenbrauker I, Kalled SL, Leitges M, Mackay F, Tarakhovsky A: Regulation of B-cell survival by BAFF-dependent PKCdelta-mediated nuclear signalling. Nature 2004;431:456–461.
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