Background: Allergy to sesame seeds is an emerging food allergy of a serious nature due to a high risk of systemic anaphylaxis. Although a mouse model to study sesame anaphylaxis is desirable, currently it is not available. Here, using a transdermal exposure model system, we tested the hypothesis that sesame seed elicits IL-4-associated IgE antibody response with consequent clinical sensitization in mice. Methods: Groups of BALB/c mice were exposed to sesame seed extract or saline or a control food (vanilla bean extract) by transdermal applications. Systemic IgE, IgG1 and IgG2a antibody responses were examined using preoptimized ELISA. Type 2 and type 1 cytokine responses were evaluated by ex vivo antigen-mediated activation of spleen cells. Clinical response to oral sesame challenge was studied. Western blot and N-terminal amino acid sequence analyses were performed to identify the sesame allergens. Results: Transdermal exposure to sesame elicited robust IgE and IgG1 but very little IgG2a antibody responses. IgE response to transdermal exposure in two high-IgE responder mice strains with disparate MHC confirmed the intrinsic allergenicity of sesame seed. Transdermal sensitization was associated with activation of IL-4 but not IFN-γ. Furthermore, oral exposure to sesame resulted in clinical signs of systemic anaphylaxis. Western blot and sequence analysis identified four allergens including Ses i 3 and the basic subunit of 11s globulins. Conclusion: These data argue that transdermal exposure to sesame seed can result in IL-4 activation, IgE response and clinical sensitization for systemic anaphylaxis.

Keywords:
Sesame, Food allergy, Cytokines, type 2, IgE
1.
Sampson HA: Update on food allergy. J Allergy Clin Immunol 2004;113:805–820.
2.
Sicherer SH, Munoz-Furlong A, Sampson HA: Prevalence of peanut and tree nut allergy in the United States determined by means of a random digit dial telephone survey: a 5-year follow-up study. J Allergy Clin Immunol 2003;112:1203–1207.
3.
Gangur V, Kelly C, Navuluri L: Sesame allergy: a growing food allergy of global proportions? Ann Allergy Asthma Immunol 2005;95:4–11, 11–13, 44.
4.
Available at: http://www.hc-sc.gc.ca/food-aliment/fsa-esa/allergen_paper/e_allergen_paper-03.html; accessed Health Canada and the Canadian Food Inspection Agency, October 13, 2004.
5.
Goodwin PR: Food allergen testing. Food Qual 2004;April/May:26–35.
6.
Birmingham N, Gangur V, Samineni S, Navuluri L, Kelly C: Hazelnut allergy: evidence that hazelnut can directly elicit specific IgE antibody response via activating type 2 cytokines in mice. Int Arch Allergy Immunol 2005;137:295–302.
7.
Li XM, Schofield BH, Huang CK, Kleiner GI, Sampson HA: A murine model of IgE-mediated cow’s milk hypersensitivity. J Allergy Clin Immunol 1999;103:206–214.
8.
Li XM, Serebrisky D, Lee SY, Huang CK, Bardina L, Schofield BH, Stanley JS, Burks AW, Bannon GA, Sampson HA: A murine model of peanut anaphylaxis: T- and B-cell responses to a major peanut allergen mimic human responses. J Allergy Clin Immunol 2000;106:150–158.
9.
Untersmayr E, Scholl I, Swoboda I, Beil WJ, Forster-Waldl E, Walter F, Riemer A, Kraml G, Kinaciyan T, Spitzauer S, Boltz-Nitulescu G, Scheiner O, Jensen-Jarolim E: Antacid medication inhibits digestion of dietary proteins and causes food allergy: a fish allergy model in BALB/c mice. J Allergy Clin Immunol 2003;112:616–623.
10.
Alday E, Curiel G, Lopez-Gil MJ, Carreno D, Moneo I: Occupational hypersensitivity to sesame seeds. Allergy 1996;51:69–70.
11.
Keskinen H, Ostman P, Vaheri E, Tarvainen K, Grenquist-Norden B, Karppinen O, Nordman H: A case of occupational asthma, rhinitis and urticaria due to sesame seed. Clin Exp Allergy 1991;21:623–624.
12.
Lack G, Fox D, Northstone K, Golding J: Factors associated with the development of peanut allergy in childhood. N Engl J Med 2003;348:977–985.
13.
Wang LF, Lin JY, Hsieh KH, Lin RH: Epicutaneous exposure of protein antigen induces a predominant Th2-like response with high IgE production in mice. J Immunol 1996;156:4077–4082.
14.
Birmingham N, Payankaulam S, Thanesvorakul S, Stefura B, HayGlass K, Gangur V: An ELISA-based method for measurement of food-specific IgE antibody in mouse serum: an alternative to the passive cutaneous anaphylaxis assay. J Immunol Methods 2003;275:89–98.
15.
Adel-Patient K, Bernard H, Ah-Leung S, Creminon C, Wal JM: Peanut- and cow’s milk-specific IgE, Th2 cells and local anaphylactic reaction are induced in Balb/c mice orally sensitized with cholera toxin. Allergy 2005;60:658–664.
16.
Hsieh KY, Tsai CC, Wu CH, Lin RH: Epicutaneous exposure to protein antigen and food allergy. Clin Exp Allergy 2003;33:1067–1075.
17.
Li XM, Srivastava K, Huleatt JW, Bottomly K, Burks AW, Sampson HA: Engineered recombinant peanut protein and heat-killed Listeria monocytogenes coadministration protects against peanut-induced anaphylaxis in a murine model. J Immunol 2003;170:3289–3295.
18.
Finotto S, Glimcher L: T cell directives for transcriptional regulation in asthma. Springer Semin Immunopathol 2004;25:281–294.
19.
Romagnani S: Immunologic influences on allergy and the TH1/TH2 balance. J Allergy Clin Immunol 2004;113:395–400.
20.
Oettgen HC, Martin TR, Wynshaw-Boris A, Deng C, Drazen JM, Leder P: Active anaphylaxis in IgE-deficient mice. Nature 1994;370:367–370.
21.
Dearman RJ, Kimber I: Determination of protein allergenicity: studies in mice. Toxicol Lett 2001;120:181–186.
22.
Scholl I, Untersmayr E, Bakos N, Roth-Walter F, Gleiss A, Boltz-Nitulescu G, Scheiner O, Jensen-Jarolim E: Antiulcer drugs promote oral sensitization and hypersensitivity to hazelnut allergens in BALB/c mice and humans. Am J Clin Nutr 2005;81:154–160.
23.
Beyer K, Bardina L, Grishina G, Sampson HA: Identification of sesame seed allergens by 2-dimensional proteomics and Edman sequencing: seed storage proteins as common food allergens. J Allergy Clin Immunol 2002;110:154–159.
24.
Asero R, Mistrello G, Roncarolo D, Antoniotti PL, Falagiani P: A case of sesame seed-induced anaphylaxis. Allergy 1999;54:526–527.
25.
Fremont S, Zitouni N, Kanny G, Veneri V, Metche M, Moneret-Vautrin DA, Nicolas JP: Allergenicity of some isoforms of white sesame proteins. Clin Exp Allergy 2002;32:1211–1215.
26.
Kolopp-Sarda MN, Moneret-Vautrin DA, Gobert B, Kanny G, Brodschii M, Bene MC, Faure GC: Specific humoral immune responses in 12 cases of food sensitization to sesame seed. Clin Exp Allergy 1997;27:1285–1291.
27.
Wolff N, Cogan U, Admon A, Dalal I, Katz Y, Hodos N, Karin N, Yannai S: Allergy to sesame in humans is associated primarily with IgE antibody to a 14 kDa 2S albumin precursor. Food Chem Toxicol 2003;41:1165–1174.
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2006
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