Background: An association between Helicobacter pylori and chronic urticaria has been suspected previously. An IgE-mediated pathway might be a possible link between H. pylori infection and chronic urticaria, and therefore we wanted to prepare an optimal H. pylori antigen to detect H. pylori-specific IgE antibodies in chronic urticaria patients. Methods:H. pylori antigen extracts were prepared in different ways to find the optimal antigen extract to be used in the assays. Immunoblotting was used to detect IgE-binding bands. The results were applied in an H. pylori RAST assay for specific H. pylori IgE antibodies in patient sera. Results: In immunoblotting, the largest number of IgE-stained bands were visualized in the washing fluids and sonicated extracts, while strong heating and denaturing treatments destroyed the epitopes for IgE binding, suggesting that they belonged to the flagellar structures of H. pylori. However, in H. pylori-specific RAST analysis, specific IgE was found only in 1 of 25 H. pylori-infected patients. Conclusions: Our findings suggest that although IgE-binding epitopes were found in H. pylori, H. pylori-specific IgE antibodies are not common in chronic urticaria, and the clinical significance of the IgE response is unclear.

Bazillou M, Fendri C, Castel O, Ingrand P, Fauchere JL: Serum antibody response to the superficial and released components of Helicobacter pylori. Clin Diagn Lab Immunol 1994;3:310–317.
Karvar S, Karch H, Frosch M, Burhardt W, Gross U: Use of serum-specific immunoglobulins A and G for detection of Helicobacter pylori infection in patients with chronic gastritis by immunoblot analysis. J Clin Microbiol 1997;35:3058–3061.
Mayo K, Pretolani S, Gasbarrini G, Ghironzi G, Megraud F: Heterogeneity of immunoglobulin G response to Helicobacter pylori measured by the unweighted pair group method with averages. Clin Diagn Lab Immunol 1998;5:50–73.
Tebbe B, Geilen CC, Schulzke JD, Bojarski C, Radenhausen M, Orfanos CE: Helicobacter pylori infection and chronic urticaria. J Am Acad Dermatol 1996;34:685–686.
Wustlich S, Brehler R, Luger TA, Pohle T, Domschke W, Foerster E: Helicobacter pylori as a possible bacterial focus of chronic urticaria. Dermatology 1999;198:130–132.
Aceti A, Celestino D, Caferro M, Casale V, Citarda F, Conti EM, Grassi A, Grilli A, Pennica A, Sciarretta F, Leri O, Ameglio F, Sebastiani A: Basophil-bound and serum immunoglobulin E directed against Helicobacter pylori in patients with chronic gastritis. Gastroenterology 1991;101:131–137.
Studenikin MY, Nijevitch AA, Mutalov AG: Consideration of Helicobacter pylori infection in childhood: Immune response, endoscopic and morphological findings. Acta Paediatr Jpn 1995;37:551–556.
Permin H, Norgaard A, Norn S, Andersen LP, Nielsen H: IgE-mediated immune response to Helicobacter pylori examined by basophil histamine release in patients with dyspepsia. Inflamm Res 2000;49:29–30.
Liutu M, Kalimo K, Uksila J, Kalimo H: Etiologic aspects of chronic urticaria. Int J Dermatol 1998;37:515–519.
Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680–685.
Towbin HT, Staehelin T, Gordon J: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci USA 1979;76:4350–4354.
Bengtsson A, Rolfsen W, Einarsson R: Characterization of allergens and patient sera by a nitrocellulose immunoprint technique. Int Arch Allergy Appl Immunol 1985;78:139–144.
Walsh BJ, Sutton R, Wrigley CW, Baldo BA: Allergen discs prepared from nitrocellulose: Detection of IgE binding to soluble and insoluble allergens. J Immunol Methods 1984;73:139–145.
Marshall BJ, Warren JR: Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet 1984;i:1311–1314.
Brown WR, Borthistle BK, Chen ST: Immunoglobulin E (IgE) and IgE-containing cells in human gastrointestinal fluids and tissues. Clin Exp Immunol 1975;20:227–237.
Andre C, Moulinier B, Andre F, Daniere S: Evidence for anaphylactic reactions in peptic ulcer and varioliform gastritis. Ann Allergy 1983;51:325–328.
Savolainen J, Viander M, Koivikko A: Distribution of watersoluble antigens and allergens of Candida albicans in blastospore cell extract fractions. Allergy 1990;45:47–53.
Figura N, Perrone A, Gennari C, Orlandini G, Giannace R, Lenzi C, Vagliasindi M, Bianciardi L, Rottoli P: CagA-positive Helicobacter pylori infection may increase the risk of food allergy development. J Physiol Pharmacol 1999;50:827–831.
Corrado G, Luzzi I, Pacchiarotti C, Lucarelli S, Frediani T, Cavaliere M, Rea P, Cardi E: Helicobacter pylori seropositivity in children with atopic dermatitis as sole manifestation of food allergy. Pediatr Allergy Immunol 2000;11:101–105.
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.