The purpose of this study was to evaluate the strain-related differences in tracheal hyperresponsiveness in control and egg albumen-sensitized guinea pigs. Concentration-response curves to acetylcholine and barium chloride were established from tracheal rings of Dunkin-Hartley and BEA strain guinea pigs. In the Dunkin-Hartley strain, sensitization did not signficantly increase the tracheal responsiveness to acetylcholine and barium chloride. By contrast, in the BFA strain, significant sensitization-induced hyperreactivity was achieved as the maximal contractions induced by acetylcholine and barium chloride, were enhanced from 6.5 ± 1.2 and 3.2 ± 0.4 mN in control to 10.0 ± 1.4 and 5.6 ± 0.8 mN, respectively, in sensitized animals. However, antigen challenge, performed in vitro, exhibited a similar amplitude of contraction in tracheal rings from both strains (Dunkin-Hartley 5.1+0.8 mN; BFA 5.9 ± 0.5 mN). Finally, while the two guinea-pig strains developed specific sensitization to allergen, only tracheal rings from the BFA strain developed hyperresponsiveness to acetylcholine and barium chloride. The strain-related difference appears to be partly explained by a lower basal reactivity in the BFA strain both to acetylcholine (Em 7.3 ± 1.7 and 6.5 ± 1.2 mN for Dunkin-Hartley and BFA, respectively) and barium chloride (Em 9.4 ± 2.6 and 3.2 ± 0.4 mN for Dunkin-Hartley and BFA, respectively). As the same procedure of sensitization provides different results in the genesis of hyperreactivity between the two guinea-pig strains used for asthma models, the BFA guinea-pig strain seems to be a better model because sensitized non-challenged animals could easily be dissociated from control ones, similar to that which occurs in asthmatic patients during provocation tests with cholinergic drugs.

Keywords:
Strain difference, Hyperreactivity, Trachea, guinea pig, Asthma, animal model
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© 1995 S. Karger AG, Basel
1995
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