We studied the binding of protein A and protein G to human monoclonal and polyclonal IgE. An ELISA was used to measure human IgE. Protein A-Sepharose did not bind to monoclonal IgE, but bound to 12–14% of serum polyclonal IgE and to ––3% of the polyclonal IgE purified from the serum. No difference in binding to protein A was found between IgE from an allergic patient and that from a non-allergic subject. Protein G bound to neither polyclonal IgE nor monoclonal IgE. Thus, protein G is better than protein A with respect to separation of IgE from IgG.

Keywords:
Protein A, Protein G, IgE, human
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© 1994 S. Karger AG, Basel
1994
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