Objective and Method: We studied the effects of human growth hormone (hGH) on leptin production and lipolysis stimulation in the presence or absence of human growth hormone binding protein (hGHBP) using 3T3- L1-hGHR adipocytes which efficiently express human growth hormone receptor. Results and Conclusion: It was clarified that (1) hGH decreases leptin secretion after hGH-induced lipolysis stimulation, and (2) the reduction of leptin production and lipolysis stimulation by 22K hGH was attenuated with hGHBP, whereas that by 20K hGH, which is a naturally occurring isoform of 22K hGH, was not affected with hGHBP.

Keywords:
Leptin, Lipolysis, hGHBP, 22K hGH, 20K hGH, 3T3-L1-hGHR adipocytes
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© 2001 S. Karger AG, Basel
2001
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