Transgenic mice which specifically express the human insulin gene in the pancreatic β cells were obtained by micro injection of DNA into fertilized one-cell embryos. The elements regulating in vivo the β-cell specificity of the insulin gene, located within a few hundred base pairs upstream to the start site of transcription, were used to construct hybrid genes expressed in pancreatic β cells. Expression of SV40-T large antigen resulted in β-cell proliferation and β-cell tumors, while major histocompatibility, or interferon-gamma molecules induced diabetes mellitus in transgenic mice.

Keywords:
Human insulin gene, Insulin gene regulation, Insulinoma, Diabetes, MHC I, MHC II
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© 1989 S. Karger AG, Basel
1989
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