Background: Ovarian tissue cryopreservation by vitrification is an attractive technique for fertility preservation in women. However, this technique has not been optimized. The aim of this study was to evaluate the baboon as a model for the preclinical study of ovarian tissue cryopreservation by vitrification and thawing. Methods: Ovarian cortical tissues (1-mm cubes) were obtained surgically from adult female olive baboons (n = 9) maintained in captivity and vitrified using dimethyl sulphoxide and ethylene glycol protocol. The proportion of morphologically intact follicles (primordial, primary and secondary) in paired fresh and cryopreserved (vitrified-thawed) ovarian tissues was compared. Results: Overall, 67.1% of follicles were morphologically normal after vitrification. When compared to fresh ovarian tissue, vitrified-thawed ovarian tissue contained a comparable number of intact primordial follicles (48.9 vs. 52.9%), and a lower number of both primary (14.8 vs. 29.5%; p < 0.05) and secondary (2.0 vs. 0.7%; p < 0.05) follicles. Conclusion: After vitrification and thawing, baboon ovarian tissue retains about 67% of morphologically normal follicles, which is comparable to results for human ovarian tissue, and suggests that the olive baboon is a promising animal model for preclinical assessment of ovarian vitrification, thawing and autotransplantation studies.

Keywords:
Vitrification, Ovarian tissues, Cryopreservation, Fertility, Baboon
1.
Blatt J: Pregnancy outcome in long-term survivors of childhood cancer. Med Pediatr Oncol 1999;33:29-33.
2.
Isachenko V, Montag M, Isachenko E, Katkov II, Van der Ven H: A new method for in vitro culture of vitrified human ovarian tissue (abstract 117). Cryobiology 2006;53:174.
3.
Meirow D, Nugent D: The effects of radiotherapy and chemotherapy on female reproduction. Hum Reprod Update 2001;7:535-543.
4.
von Wolff M, Donnez J, Hovatta O, Keros V, Maltaris T, Montag M, Salle B, Sonmezer M, Andersen CY: Cryopreservation and autotransplantation of human ovarian tissue prior to cytotoxic therapy - a technique in its infancy but already successful in fertility preservation. Eur J Cancer 2009;45:1547-1553.
5.
Wang Y, Xiao Z, Li L, Fan W, Li SW: Novel needle immersed vitrification: a practical and convenient method with potential advantages in mouse and human ovarian tissue cryopreservation. Hum Reprod 2008;23:2256-2265.
6.
Donnez J, Dolmans MM, Demylle D, Jadoul P, Pirard C, Squifflet J, Martinez-Madrid B, van Langendonckt A: Livebirth after orthotopic transplantation of cryopreserved ovarian tissue. Lancet 2004;364:1405-1410.
7.
Meirow D, Levron J, Eldar-Geva T, Hardan I, Fridman E, Zalel Y, Schiff E, Dor J: Pregnancy after transplantation of cryopreserved ovarian tissue in a patient with ovarian failure after chemotherapy. N Engl J Med 2005;353:318-321.
8.
Varghese AC, du Plessis SS, Falcone T, Agarwal A: Cryopreservation/transplantation of ovarian tissue and in vitro maturation of follicles and oocytes: challenges for fertility preservation. Reprod Biol Endocrinol 2008;6:47.
9.
Demeestere I, Simon P, Emiliani S, Delbaere A, Englert Y: Fertility preservation: successful transplantation of cryopreserved ovarian tissue in a young patient previously treated for Hodgkin's disease. Oncologist 2007;12:1437-1442.
10.
Andersen CY, Rosendahl M, Byskov AG, Loft A, Ottosen C, Dueholm M, Schmidt KL, Andersen AN, Ernst E: Two successful pregnancies following autotransplantation of frozen/thawed ovarian tissue. Hum Reprod 2008;23:2266-2272.
11.
Lee DM, Yeoman RR, Battaglia DE, Stouffer RL, Zelinski-Wooten MB, Fanton JW, Wolf DP: Live birth after ovarian tissue transplant. Nature 2004;428:137-138.
12.
Gosden RG, Baird DT, Wade JC, Webb R: Restoration of fertility to oophorectomized sheep by ovarian autografts stored at -196 degrees C. Hum Reprod 1994;9:597-603.
13.
Parrot DM: Fertility of mice with orthotopic ovarian grafts derived from frozen tissue. J Reprod Fertil 1960;1:230-241.
14.
Wang X, Chen H, Yin H, Kim SS, Lin Tan S, Gosden RG: Fertility after intact ovary transplantation. Nature 2002;415:385.
15.
Li YB, Zhou CQ, Yang GF, Wang Q, Dong Y: Modified vitrification method for cryopreservation of human ovarian tissues. Chin Med J (Engl) 2007;120:110-114.
16.
Tokieda Y, Ishiwata I, Segino M, Ishikawa H, Sato K: Establishment of a novel method for cryopreservation and thawing of the mouse ovary. Hum Cell 2002;15:230-237.
17.
Migishima F, Suzuki-Migishima R, Quintero RB, Yokoyama M, Behr BR: Successful pregnancies after transplantation of frozen-thawed mouse ovaries into chimeric mice that received lethal-dose radiation. Fertil Steril 2006;86(suppl 4):1080-1087.
18.
Dudzinski DM: Ethical issues in fertility preservation for adolescent cancer survivors: oocyte and ovarian tissue cryopreservation. J Pediatr Adolesc Gynecol 2004;17:97-102.
19.
Yeoman RR, Wolf DP, Lee DM: Coculture of monkey ovarian tissue increases survival after vitrification and slow-rate freezing. Fertil Steril 2005;83(suppl 1):1248-1254.
20.
Stevens VC: Some reproductive studies in the baboon. Hum Reprod Update 1997;3:533-540.
21.
Nyachieo A, Spiessens C, Chai DC, Mwenda JM, D'Hooghe TM: Menstrual cycle synchronization, ovarian stimulation, and in vitro fertilization in olive baboons (Papio anubis): a prospective randomized study. Fertil Steril 2009;91:602-610.
22.
D'Hooghe TM, Bambra CS, Cornillie FJ, Isahakia M, Koninckx PR: Prevalence and laparoscopic appearance of spontaneous endometriosis in the baboon (Papio anubis, Papio cynocephalus). Biol Reprod 1991;45:411-416.
23.
D'Hooghe TM, Nyachieo A, Chai DC, Kyama CM, Spiessens C, Mwenda JM: Reproductive research in non-human primates at Institute of Primate Research in Nairobi, Kenya (WHO Collaborating Center): a platform for the development of clinical infertility services? Hum Reprod, DOI:10.1093/humrep/den164.
24.
Nyachieo A, Chai DC, Deprest J, Mwenda JM, D'Hooghe TM: The baboon as a research model for the study of endometrial biology, uterine receptivity and embryo implantation. Gynecol Obstet Invest 2007;64:149-155.
25.
Vanderzwalmen P, Bertin G, Debauche Ch, Standaert V, Bollen N, van Roosendaal E, Vandervorst M, Schoysman R, Zech N: Vitrification of human blastocysts with the Hemi-Straw carrier: application of assisted hatching after thawing. Hum Reprod 2003;18:1504-1511.
26.
Paynter SJ, Cooper A, Fuller BJ, Shaw RW: Cryopreservation of bovine ovarian tissue: structural normality of follicles after thawing and culture in vitro. Cryobiology 1999;38:301-309.
27.
Nichols SM, Bavister BD, Brenner CA, Didier PJ, Harrison RM, Kubisch HM: Ovarian senescence in the rhesus monkey (Macaca mulatta). Hum Reprod 2005;20:79-83.
28.
Gook DA, Edgar DH, Stern C: Effect of cooling rate and dehydration regimen on the histological appearance of human ovarian cortex following cryopreservation in 1, 2-propanediol. Hum Reprod 1999;14:2061-2068.
29.
Schmidt KL, Byskov AG, Andersen AN, Muller J, Andersen CY: Density and distribution of primordial follicles in single pieces of cortex from 21 patients and in individual pieces of cortex from three entire human ovaries. Hum Reprod 2003;18:1158-1164.
30.
Smitz J: Oocyte development competence after heterotopic transplantation of cryopreserved ovarian tissue. Lancet 2004;363:832-833.
31.
West ER, Zelinski MB, Kondapalli LA, Gracia C, Chang J, Coutifaris C, Critser J, Stouffer RL, Shea LD, Woodruff TK: Preserving female fertility following cancer treatment: current options and future possibilities. Pediatr Blood Cancer 2009;53:289-295.
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2013
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