... in lysosomes. The biosynthetic rate of subunit c and mRNA levels for P1 and P2 genes that code for it were almost the same in both control and patient cells. Measurement of labeled subunit c in mitochondrial and lysosomai fractions showed a specific delay of degradation of subunit c in patient cells with late...

... of its autofluorescence. Cells exposed to peroxidized POS accumulated much more lipofuscin than those exposed to native POS, indicating that peroxidized outer segements are not digestable by lysosomal enzymes. Furthermore, lipofuscin-loaded RPE cells were considerable more sensitive to visible blue light...

... determined the degree of AV accumulation 4 h after the injection of vinblastine, which blocks the fusion of AV with lysosomes. Other animals were treated with Triton X-100, which provokes the appearance of AV, and then, after 4 h, with cycloheximide to block the appearance of newly formed AV. AV elimination...

... decreased from 14 to 24 mo. These results suggest that the increase in lipofuscin with age may not be due to an age-wise decline in cathepsin B activity. 15 4 2009 Age-pigment Post-mitotic cells Thiol proteases Lysosomes Histochemistry © 1995 S. Karger AG, Basel 1995 Copyright...

... a discontinuous gradient of metrizamide preceded by a low-speed centrifugation. An investigation was carried out to compare subcellular fractions prepared from early-passage and senescent cells. Proteolytic activity associated with lysosomes and soluble proteolytic activity were found to be increased in senescent...

...Brigitte Hermelin; Jacques Picard Properties of two hydrolases: β -glucuronidase and N-acetyl- β -hexosaminidase were studied in lysosomal fractions of media-intima from arterial wall. These enzymatic activities change significantly with aging. In the arterial wall, the decrease in activities of β...

...Harold H. Traurig The activities of four lysosomal acid hydrolases (LAH) in the lungs of two strains of mice changed significantly throughout the life cycle. In the C57B1/6J animals, acid phosphatase (AP) and β- glucuronidase (β-G) were maximally active during early neonatal life then gradually...