The most common form of sensory disability is age-related hearing loss (ARHL), also referred to as presbycusis. ARHL is a complex disorder with a mixture of genetic and environmental components, a combination that leads to a progressive decline in hearing function with increased age. In the last 15 years, there has been a vast increase in our knowledge of the genes that underlie congenital deafness and the critical components of hearing. In contrast, knowledge of the pathological processes involved in ARHL remains very limited. The mouse has proved an essential tool in the identification of early-onset deafness genes and in revealing the basic mechanisms of hearing. As focus is now turning toward elucidating the most common form of hearing loss, ARHL, the mouse will again play a fundamental role in this research. Here, we review the need for an animal model and discuss the suitability of the mouse as an ARHL model. Finally, we outline the ways in which hearing researchers are utilising the mouse in the investigation of ARHL and provide perspectives on the need for these data to be integrated with the results of human genetic studies.

An age-dependent, progressive decline in hearing function is so common that it is often mistakenly perceived as an inevitable part of the ageing process in humans. It is partly as a consequence of this perception that the overall contribution of hearing loss to disability and reduced quality of life is under-appreciated. However, in the context of increasing longevity of the population, the contribution of hearing loss to the general health and well-being of individuals during that extended lifespan is of great clinical and economic significance [1]. Age-related hearing loss (ARHL) is the most common sensory loss in the elderly. The frequency of bilateral hearing loss doubles for every 10 years over the age of 50. Thus, 15% of people between 50 and 59 years have bilateral hearing loss, rising to 31% in people 60-69 years of age and 63% in those over 70 years (US National Health and Nutrition Examination Survey [2]). Projections in both the US and UK suggest that the number of people affected by ARHL will effectively double by 2031, largely due to an increased lifespan [2] (Action on Hearing Loss http://www.action ARHL inevitably causes communication difficulties and is associated with social isolation, depression and reduced physical and cognitive function [3,4]. There is a growing literature on the association between hearing loss and cognitive decline; however, it is unknown whether this link is via common pathological mechanisms or because the lack of auditory input contributes to accelerated cognitive decline [5]. Hearing aids are currently the only treatment option for ARHL. In this context, research into the causes and pathology that underlie ARHL is greatly needed in order to develop therapeutic strategies and treatments.

Research to reveal the causes of ARHL faces a number of significant challenges. First, the auditory system is highly complex. An indication of the high number of critical components in this sensory organ is shown by the number of genes in which mutations can cause congenital deafness; over 150 genetic loci have been mapped, and it is likely that ARHL will be at least as heterogeneous, if not more so [6]. In order to detect and discriminate among sound levels across a range of frequencies, the auditory system has a sophisticated, tonotopic organisation that needs to be maintained from the cochlea to the brain. There are 26 different cell types just within the cochlea, each with a specialised function and arranged into an intricate and highly organised structure (fig. 1a-c). Within this structure, there are the mechanical transducers, the sensory hair cells. Progressive loss of these cells throughout life is associated with ARHL, typically beginning in the higher frequencies due to loss of hair cells in the basal turn of the cochlea and later progressing to the apical turn, affecting lower frequencies. However, it is still unclear whether this hair cell loss is the underlying cause of ARHL or is a result of pathology that originates elsewhere in the auditory system and eventually leads to hair cell death. Schuknecht's seminal work on the pathology of ARHL, beginning in the 1950s, proposed four pathological subtypes in presbycusis: sensory, involving hair cell loss (fig. 1d); strial (or metabolic), involving degeneration of the stria vascularis and reduction in endocochlear potential; neural, involving loss of spiral ganglion neurons (SGNs; fig. 1e), and mechanical, involving stiffening of the basement membrane, although the latter is now thought to be a manifestation of strial presbycusis [7,8,9]. Evidence suggests a mixed pathology of these different subtypes is present in most people with ARHL, indicating that a number of different pathways contribute to the hearing loss [8,9].

In addition to the complexity of the system, any biochemical analysis of the cochlea is compromised because removal of intact human cochlear tissue for functional characterisation, even post-mortem, is confounded by the cochlea being embedded within the temporal bone, the hardest bone in the body. In this scenario, a genetic approach is often deployed because it does not rely on biological knowledge or access to affected tissue. However, as with other highly heterogeneous and complex disorders, genome-wide association (GWA) studies into adult hearing function have had very limited success in identifying ARHL susceptibility genes. To date, five GWA studies have been performed and none has identified a genetic association above the level of statistical significance sufficient for such genome-wide studies. Nevertheless, these studies do provide a valuable candidate gene list for further investigation. The first GWA study into ARHL by Friedman et al. [10] reported an association between single nucleotide polymorphisms within the GRM7 gene and ARHL, which - although not above genome wide significance - was replicated in some cohorts, an important criterion in validating genetic associations. The second by Van Laer et al. [11], employing samples from the isolated Finnish Saami population, revealed no significant GWAs for ARHL. The third by Girotto et al. [12] utilised a meta-analysis approach to study normal hearing traits in adults, employing samples from 6 isolated European populations. This study detected 8 suggestive loci in genes expressed in the inner ear, including DCLK1, PTPRD, CMIP and GRM8 (a homologue of GRM7), as well as loci containing genes of unknown function or genes whose function has not been linked to hearing. The fourth GWA study was undertaken into the hearing status of 3,614 individuals (all aged 44-45 years) in the British 1958 Birth Cohort and again failed to find a significant association but provided the basis for the subsequent characterisation of a novel hearing gene, ESRRG, in a knock-out mouse model [13]. The fifth by Fransen et al. [14] is an extension of the first GWA study, employing a larger sample set. This recent study revealed no significant GWAs for ARHL and also did not add support to any of the previously reported associations in GRM7 or GRM8. Despite these disappointing outcomes, it is likely that these studies will provide a valuable candidate gene set for deciphering the molecular mechanisms that can cause ARHL, but they require secondary validation through functional assessment for a role in hearing loss [10,12,13]. In summary, the biologist, cell physiologist and geneticist all face particular challenges in human ARHL research, meaning the need for animal models to help identify and characterise ARHL genes, and to corroborate genetic findings in humans is of utmost importance.

Mice continue to be the predominant model organism for hearing research. Similarities in the auditory structure and physiology between mice and humans, the close evolutionary relationship of genomes (∼99% of genes in mice have a human orthologue), relatively low housing costs (small size, short lifespan), genetic standardisation and the available genetic toolkit together make the mouse a useful and crucial model system for studying the functional genomics of the auditory system [15,16,17]. The ease at which mice can be genetically manipulated has led to the generation of numerous mouse models that mimic human hearing loss mutations, allowing the study of gene function. Additionally, the ability to screen large numbers of mice for hearing defects by means of a simple click box test meant that the hearing field was an early adopter of mutant mouse technology. Hence, in addition to gene-driven approaches, phenotype-driven screens of chemically induced mouse mutants have led to the identification of many novel deafness genes, which have subsequently helped to increase our knowledge and understanding of the genetic bases of human hearing impairment [15,16,18]. Critically, characterisation of these mouse models has allowed the relationship between genetic variants and their consequent structural and pathological changes within the ear to be explored, something that is rarely possible in humans. The high-throughput phenotyping of spontaneous, genetic and chemical-induced mutants for hearing loss, and the subsequent mapping and identification of the mutated genes has provided the basis for our current understanding of the key players in the auditory system. This phenotype-driven paradigm, which has proven so successful in mapping hearing genes, has also been implemented for the discovery of disease genes in other disciplines [19].

Animal models are commonly used to investigate the genetic and physiological basis of ageing and age-related disease. They are required because the study of ageing in humans is complicated by several factors, including longevity, accumulated environmental influences, genetic heterogeneity and ethical issues. In particular, the laboratory mouse has become a very robust and reliable tool for ageing research, not least because of the ability to strictly control both intrinsic and extrinsic factors, e.g. genetic background, diet, environment and health status [20]. In addition, the mouse is a practicable and useful model because they have a short lifespan, meaning the effects of age are apparent within a contracted time period, and tissues can be obtained and analyzed at all stages of the ageing process [21].

As previously mentioned, mouse mutants have been instrumental in the discovery of genes that are involved in congenital and early-onset hearing loss. However, utilisation of the mouse for the study of ARHL has mainly focused on inbred strains, as it has long been known that certain strains exhibit very different ageing characteristics [22]. In addition, certain inbred strains display an innate propensity to noise-induced hearing loss [23,24]. These strain-specific traits indicate a genetic basis for these differences, and consequently inbred mice have been used to elucidate genetic loci and pathology associated with ARHL and noise-induced hearing loss (table 1). To date, almost 20 loci contributing to ARHL have been defined ( However, the underlying genes have been identified for only four of these ARHL (ahl) loci, and they are Cdh23 (ahl1), Cs (ahl4), Gipc3 (ahl5) and Fscn2 (ahl8) [25,26,27,28]. Interestingly, three out of four of the identified genes (Cdh23, Gipc3 and Fscn2) encode proteins that are reported to be important for hair cell stereocilium structure and/or function (table 1). This reinforces Schuknecht's premise that defects in these sensory cells contribute to presbycusis. The Cdh23ahl allele is defined by the presence of an A rather than a G at the last position of the 7th coding exon. This hypomorphic allele causes an increased frequency of in-frame exon skipping and is common to at least 10 inbred strains with increased susceptibility to ARHL [22]. Cdh23 encodes a cell-cell adhesion glycoprotein expressed in the sensory epithelium of the inner ear and is required for stereocilium bundle formation and function. Mutant alleles of the human orthologue CDH23 cause recessive deafness (DFNB12) and Usher's syndrome (USH1D). The widely used and much studied C57BL/6J inbred strain has the Cdh23ahl allele [22]. This strain begins to display elevated hearing thresholds from as early as 3-6 months of age, starting in the high frequencies, then the low, before leading to a profound hearing loss across all frequencies. Given the expression pattern observed for this protein, it is unsurprising that hearing loss is associated with hair cell degeneration, a feature of sensory ARHL. This includes progressive loss of both inner and outer hair cells in an age-dependent manner proceeding from the base to the apex of the cochlear spiral, with outer hair cell loss observed at the base of the cochlea by 3 months of age [29]. Between 3 and 7 months of age, loss of inner hair cells becomes evident at the base of the cochlear spiral. By 7 months of age, loss of spiral ganglion cells is also evident at the basal turn and is consistent with a secondary neuronal degeneration subsequent to inner hair cell loss [29]. Additionally, age-related changes are also seen in the spiral ligament and stria vascularis consisting of fibrocyte loss and thinning, respectively, although a direct causal link between these pathologies has yet to be determined [21]. Together, these pathological changes affect cochlear structures that match with the main ARHL subtypes proposed by Schuknecht (sensory, neural and strial), the exception being that even with the broad cellular degeneration seen in these mice, they do not show a decline in endocochlear potential [21]. Furthermore, C57BL/6J mice have been employed to investigate the role of melanin pigment, expressed by the intermediate cells of the stria vascularis, as a protective factor against ARHL and noise-induced hearing loss. The study utilised two co-isogenic C57BL/6J substrains: C57BL/6J, which shows very little age-related endocochlear potential decline, and C57BL/6J-Tyrc-2J, which does not produce any strial melanin due to a naturally occurring mutation in the tyrosinase locus. Assessment of these mice showed identical rates of hearing loss and sensory cell loss. However, when aged (>2 years), the C57BL/6J-Tyrc-2J mice have reduced endocochlear potentials compared to the pigmented C57BL/6J mice, and this correlates with a significantly reduced strial thickness in the albino mice due to a net loss of marginal cells [30]. The authors propose that absence of melanin may represent one of many genetic and environmental factors that promote age-related strial marginal cell loss perhaps through a reduced ability to scavenge reactive oxygen species (ROS), a known property of melanin. Anecdotally, while ethnic differences in hearing sensitivity are not well understood, African Americans have better pure-tone thresholds and a lower prevalence of hearing impairment than white or Hispanic Americans [2].

In addition to age-related sensory and strial changes in the inner ear, progressive loss of SGNs can lead to neural ARHL, and while commonly observed in humans and mice, little is known of the underlying processes. However, as with other forms of ARHL, the study of inbred and genetically engineered mutant lines is helping to address this. In particular, there are several mouse models that display sensorineural hearing loss caused by defects in mitochondrial function, e.g. Cs, Gpx1, mt-Tr, Polg1 and Sod1 (table 1). Indeed, mitochondrial pathology is associated with both inherited and acquired hearing loss. Inherited mitochondrial DNA (mtDNA) point mutations and deletions have been implicated in both syndromic and isolated sensorineural hearing loss, whereas a high frequency of acquired mtDNA mutations have been reported in patients with presbycusis [31,32]. It has been hypothesised that mitochondrial dysfunction can occur as a result of oxidative stress, such that ROS produced by the mitochondria can damage critical mitochondrial components, including mtDNA and respiratory chain proteins [33]. Increased production of ROS with age likely leads to accumulation of mitochondrial damage, which in turn leads to energy depletion and eventually tissue dysfunction. Support for this comes from the study of aged mice lacking Cu/Zn superoxide dismutase (Sod1), an anti-oxidant enzyme that protects cells from ROS, which display severe SGN degeneration [34]. Similarly, mice carrying an allele of the mitochondrial Polg DNA polymerase gene that causes mutations to accumulate in mtDNA also develop progressive hearing impairment due to apoptotic loss of SGNs [35]. At a systemic level, caloric restriction has been observed to delay ARHL in some strains (e.g. CBA/J) and to ameliorate age-related SGN loss in C57BL/6J mice. These effects are thought to occur through several pathways, influencing the insulin/IGF-1 pathway, increasing mitochondrial function and regulating gene expression, for example [36]. Interestingly, mice fed a diet rich in anti-oxidants that selectively improves the mitochondrial anti-oxidant defence system prevents SGN loss and delays ARHL [37].

Investigations of inbred strains and mouse models have greatly increased our knowledge regarding the genes and pathobiology associated with ARHL (table 1). These successes prove the utility of this model organism and support the continued use of the mouse for these studies. Ultimately, it is hoped that increased understanding of the underlying genetics will lead to therapeutic opportunities to ameliorate age-related auditory decline.

Recently, Ohmen et al. [38] reported the use of GWA studies for the high-resolution mapping of loci associated with ARHL in the mouse. By combining several heterogeneous phenotypic data sets in a meta-analysis, they successfully identified five loci showing genome-wide significance. One of these confirms the previously identified ahl8 locus, and the remaining four represent novel candidate loci for ARHL in mice. Interrogation of these and previously reported loci will lead to the identification of additional genes involved in age-related auditory function in inbred mouse strains.

As mentioned previously, phenotype-driven screens of chemically induced mouse mutants have been very effective for the identification of models of hearing loss. However, given the early-onset phenotyping bias inherent in many of these screens, the majority of identified mouse models have either congenital or acute early-onset phenotypes. Extending the approach to include screening of aged mutant mice would be one way to uncover new genes associated with age-related auditory function. However, the need to house and phenotype sufficient numbers of mice to an age to detect what are mild, late-onset phenotypes requires a major economic commitment. In addition, the presence of a pre-existing genetic susceptibility to ARHL in commonly used strains of laboratory mice is a confounding factor in the development of any ARHL screen. The MRC Harwell Ageing Screen is designed to address these issues and is the first large-scale project to successfully employ N-ethyl-N-nitrosourea (ENU) mutagenesis and phenotyping to generate mouse models of age-related disease. Large recessive pedigrees are bred and enter a phenotyping pipeline comprising recurrent assessment across a wide range of disease areas, up to 18 months of age. The pipeline includes recurrent click box and auditory-evoked brainstem response phenotyping to enable the identification of models with ARHL [39]. Unlike standard screens, the large pedigrees utilised in this screen allow for genome mapping of the critical interval using only the original cohort of mice. As such, no breeding, ageing or phenotyping of additional animals is required for the identification of the causative ENU-induced mutation. To elaborate on the underlying lesion, whole genome sequencing followed by variant analysis is undertaken. This program is beginning to identify pedigrees with late-onset hearing loss phenotypes. It will be interesting to discover if these pedigrees harbour mutations in novel hearing loss genes, or if they have ‘mild' alleles of known deafness genes.

The major genetic studies that have addressed adult hearing function in humans have identified candidate gene sets for ARHL susceptibility factors. However, the lack of genome-wide significant observations in these studies and lack of replication between different studies mean they will require secondary validation by other methods to distinguish the true ARHL susceptibility genes from chance observations. With the previously mentioned difficulties in performing any such functional characterisation in human tissue, the mouse is the most viable system in which to characterise the role of these genes in the auditory system. The most immediate way in which to validate their role in hearing is by examination of the hearing function in the relevant mutant mouse model; the Esrrg knock-out mouse was recently found to have a hearing deficit when investigated after a suggestive association with adult human hearing in a genome-wide study providing evidence for a link between this gene and hearing for the first time [13]. The aim of the International Mouse Phenotyping Consortium ( to produce a functional knock-out mouse for every known gene will facilitate this process. Although its own primary phenotyping will detect early-onset hearing loss only, it will provide an opportunity for a systematic prioritisation of human GWA study genes by known hearing function in mice and an available model for further characterisation at an older age [40].

One of the most exciting recent developments in mouse research is the use of the CRISPR/Cas9 genome-editing technology, which provides a rapid and efficient way of generating mouse models [41]. In particular, this technique has the potential to enable the production of mouse mutants carrying specific gene mutations identified in patients, thus allowing disease-causing alleles to be modelled and characterised in the mouse. In the future, this approach will undoubtedly be used to test the pathogenicity of gene variants, including those identified through next-generation sequencing of families with progressive hearing loss. The facility to characterise a mouse model of a human mutation is of great benefit, as it will allow the primary site of pathology to be identified and the associated secondary pathobiological consequences to be monitored, something that is not possible in patients.

In conclusion, integrated human and mouse approaches will lead to a more profound understanding of the genetics underlying ARHL. The study and comparison of mouse models will provide essential information regarding the molecular and cellular processes occurring in the ageing mammalian cochlea. This increased understanding will lay the foundation necessary for developing preventive modalities, such as gene, anti-oxidant or stem cell therapies.

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