Research on Neotropical primates' reproduction is necessary due to the lack of available information and the increasing threat to these species. Callimico goeldii is listed as Vulnerable on the IUCN Red List of Threatened Species. This study aimed to test rectal electrostimulation for semen collection and evaluate seminal characteristics. Therefore, semen from 6 captive Goeldi's monkeys was collected and, for the first time, seminal characteristics are described. Coagulum formation was noted in all ejaculates, and we obtained partial or complete liquefaction of the samples. Results were (means ± SD): volume = 26.9 ± 11.87 μL; pH = 7.61 ± 0.28; concentration = 143.18 ± 174.96 × 106 spermatozoa/mL; total sperm motility = 83.33 ± 5.16%; linear progressive motility = 46 ± 24.08%; plasma membrane integrity = 36.38 ± 16.11%; acrosome integrity using fast-green/bengal-rose staining = 63.41 ± 11.72%, and kit Spermac® = 69.36 ± 11.81%; abnormal sperm = 72.5 ± 17.7%, with 16.2 ± 7.7% major defects and 56.3 ± 10% minor defects; sperm with high mitochondrial activity class I = 16.45 ± 22.25%. Rectal electrostimulation was an efficient method for semen collection in this species. Investigations are required to improve semen collection and handling, including cryopreservation methods.

1.
Araújo A, De Sousa MBC (2008). Testicular volume and reproductive status of wild Callithrix jacchus. International Journal of Primatology 29: 1355-1364.
2.
Araújo LL, Lima JS, Oliveira KG, et al (2009). Uso de solução à base de água de coco a 37°C como diluidor de sêmen de Cebus apella (macaco-prego) mantido em cativeiro. Ciência Animal Brasileira 10: 588-594.
3.
Barnabe RC, Guimarães MABV, Oliveira CA, Barnabe AH (2002). Analysis of some normal parameters of the spermiogram of captive capuchin monkeys (Cebus apella Linnaeus, 1758). Brazilian Journal of Veterinary Research and Animal Science 39: 331-333.
4.
Barth AD, Oko RJ (1989). Evaluation of the spermiogram. In Abnormal Morphology of Bovine Spermatozoa (Barth AD, Oko RJ, eds.), pp 271-279. Ames, Iowa State University Press.
5.
Bennett JP (1967). Semen collection in the squirrel monkey. Journal of Reproduction and Fertility 13: 353-355.
6.
Blom E (1973). The ultrastructure of some characteristic sperm defects and a proposal for a new classification of the bull spermiogram. Nordisk Veterinaermedicin 25: 383-391.
7.
Bush DE, Russel LH, Flowers AI, Sorensen AM (1975). Semen evaluation in capuchin monkeys (Cebus apella). Laboratory Animal Science 25: 588-593.
8.
Carvalho FM, Arakaki PR, Nichi M, et al (2014). Evaluation of sperm quality in successive regular collections from captive black-and-gold howler monkeys (Alouatta caraya). Animal Reproduction 11: 11-18.
9.
Cornejo F (2008). Callimico goeldii. The IUCN Red List of Threatened Species. .
10.
Cui KH, Flaherty SP, Newble CD, Guerin M V, Napier AJ, Matthews CD (1991). Collection and analysis of semen from the common marmoset (Callithrix jacchus). Journal of Andrology 12: 214-220.
11.
Dixson AF (1998). Sexual selection and evolution of the seminal vesicles in primates. Folia Primatologica 69: 300-306.
12.
Harrison RM, Wolf RH (1985). Sperm parameters and testicular volumes in Saguinus mystax. Journal of Medical Primatology 14: 281-284.
13.
Hernández-López L, Cerda-Molina AL, Páez-Ponce DL, Mondragón-Ceballos R (2008). The seminal coagulum favours passage of fast-moving sperm into the uterus in the black-handed spider monkey. Reproduction 136: 411-421.
14.
Hernández-López L, Parra GC, Cerda-Molina AL, Pérez-Bolaños SC, Díaz Sánchez V, Mondragón-Ceballos R (2002). Sperm quality differences between the rainy and dry seasons in captive black-handed spider monkeys (Ateles geoffroyi). American Journal of Primatology 57: 35-41.
15.
Hershkovitz P (1977). Living New World Monkeys (Platyrrhini) with an Introduction to Primates. Vol 1. Chicago, University of Chicago Press.
16.
Hrudka F (1987). Cytochemical and ultracytochemical demonstration of cytochrome c oxidase in spermatozoa and dynamics of its changes accompanying ageing or induced by stress. International Journal of Andrology 10: 809-828.
17.
Hsieh M-L, Huang S-T, Huang H-C, Chen Y, Hsu Y-C (2009). The reliability of ultrasonographic measurements for testicular volume assessment: comparison of three common formulas with true testicular volume. Asian Journal of Andrology 11: 261-265.
18.
Kuederling I, Morrell JM, Nayudu PL (1996). Collection of semen from marmoset monkeys (Callithrix jacchus) for experimental use by vaginal washing. Laboratory Animals 30: 260-266.
19.
Lawrence TD, Pointdexter AN, Ritter MB, Seager SWJ, Deter RL (1976). Freeze preservation of squirrel monkey sperm for use in timed fertilization studies. Fertility and Sterility 27: 723-729.
20.
Leão DL, Miranda SA, Brito AB, Lima JS, Santos RR, Domingues SFS (2015). Efficacious long-term cooling and freezing of Sapajus apella semen in ACP-118®. Animal Reproduction Science 159: 118-123.
21.
Lundwall A (1998). The cotton-top tamarin carries an extended semenogelin I gene but no semenogelin II gene. European Journal of Biochemistry 255: 45-51.
22.
Massarotto VM, Carvalho FM, Arakaki PR, et al (2010). Collection and analysis of semen from Callithrix penicillata. Primate Research 26: 164.
23.
Moreland RB, Richardson ME, Lamberski N, Long JA (2001). Characterizing the reproductive physiology of the male southern black howler monkey, Alouatta caraya. Journal of Andrology 22: 395-403.
24.
Morrell JM, Küderling I, Hodges JK (1996). Influence of semen collection method on ejaculate characteristics in the common marmoset, Callithrix jacchus. Journal of Andrology 17: 164-172.
25.
Oliveira KG, Miranda SA, Leão DL, et al (2011). Semen coagulum liquefaction, sperm activation and cryopreservation of capuchin monkey (Cebus apella) semen in coconut water solution (CWS) and TES-TRIS. Animal Reproduction Science 123: 75-80.
26.
Poches RA, Brieva CI, Jiménez C (2013). Características seminales del tití gris (Saguinus leucopus) bajo condiciones de cautiverio obtenidas por estimulación vibratoria del pene (EVP). Revista de la Facultad de Medicina Veterinaria y de Zootecnia 60: 11-22.
27.
Pope CE, Zhang YZ, Dresser BL (1991). A simple staining method for evaluating acrosomal status of cat spermatozoa. Journal of Zoo and Wildlife Medicine 22: 87-95.
28.
Porter LM (2001). Social organization, reproduction and rearing strategies of Callimico goeldii: new clues from the wild. Folia Primatologica 72: 69-79.
29.
Porter LM, Christen A (2002). Fungus and Callimico goeldii: new insights into Callimico goeldii behavior and ecology. Evolutionary Anthropology 11: 87-90.
30.
Porter LM, Garber PA (2004). Goeldi's monkeys: a primate paradox? Evolutionary Anthropology 13: 104-115.
31.
Sampaio WV, Leão DL, Sousa PC, Domingues SFS (2017). Contribuições da biotecnologia da reprodução sobre os desafios na manipulação do sêmen de primatas do gênero Saimiri. Revista Brasileira de Reprodução Animal 41: 222-230.
32.
Sankai T, Tsuchiya H, Ogonuki N, Cho F, Yoshikawa Y (1997). A trial of oocyte maturation and in vitro fertilization by frozen-thawed spermatozoa in the red-bellied tamarin (Saguinus labiatus). Journal of Mammalian Ova Research 14: 205-208.
33.
Schneiders A, Sonksen J, Hodges JK (2004). Penile vibratory stimulation in the marmoset monkey: a practical alternative to electro-ejaculation, yielding ejaculates of enhanced quality. Journal of Medical Primatology 33: 98-104.
34.
Silva GA, Monteiro FOB, Dias HLT, et al (2013a). Qualitative analysis of preputial and vaginal bacterial microbiota in owl monkeys (Aotus azarai infulatus) raised in captivity. Journal of Medical Primatology 42: 71-78.
35.
Silva KSM, Ribeiro HFL, Valle RR, Sousa JS, Silva AO, Barbosa EM (2013b). Efeitos do trimetilaminoetano (TES) e ringer lactato em sêmen de macacos-aranha mantidos em cativeiro (Ateles paniscus e A. marginatus). Arquivos Brasileiros de Medicina Veterinária e Zootecnia 65: 934-937.
36.
Valle RR, Guimarães MABV, Muniz JAPC, Barnabe RC, Vale WG (2004). Collection and evaluation of semen from captive howler monkeys (Alouatta caraya). Theriogenology 62: 131-138.
37.
Valle RR, Valle CMR, Nichi M, Muniz JAPC, Nayudu PL, Guimarães MABV (2008). Validation of non-fluorescent methods to reliably detect acrosomal and plasma membrane integrity of common marmoset (Callithrix jacchus) sperm. Theriogenology 70: 115-120.
38.
Valle RR, Valle CMR, Nichi M, Muniz JAPC, Nayudu PL, Guimarães MABV (2014). Semen characteristics of captive common marmoset (Callithrix jacchus): a comparison of a German with a Brazilian colony. Journal of Medical Primatology 42: 225-230.
39.
Vidal FD, Luz MS, Pinho TG, Pissinatti A (2007). Coleta de sêmen em mico-leão-de-cara-dourada (Leontopithecus chrysomelas) (Kuhl, 1820) através da eletroejaculação. Callitrichidae - Primates. Revista Brasileira de Ciência Veterinária 14: 67-71.
40.
WHO (World Health Organization) (2010). WHO Laboratory Manual for the Examination and Processing of Human Semen, ed 5. Geneva, World Health Organization.
41.
Wildt D, Pukazhenthi B, Brown J, Monfort S, Howard J, Roth T (1995). Spermatology for understanding, managing and conserving rare species. Reproduction and Fertility Development 7: 811-824.
42.
Wolf DP (2009). Artificial insemination and the assisted reproductive technologies in non-human primates. Theriogenology 71: 123-129.
43.
Yeoman RR, Ricker RB, Williams LE, Sonksen J, Abee CR (1997). Vibratory stimulation of ejaculation yields increased motile spermatozoa, compared with electroejaculation, in squirrel monkeys (Saimiri boliviensis). Contemporary Topics in Laboratory Animals Science 36: 62-64.
44.
Yeoman RR, Sonksen J, Gibson SV, Rizk BM, Abee CR (1998). Penile vibratory stimulation yields increased spermatozoa and accessory gland production compared with rectal electroejaculation in a neurologically intact primate (Saimiri boliviensis). Human Reproduction 13: 2527-2531.
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