Purpose: To study the toxicity of latex urinary catheters on cultured human urothelial cells (HUC).Materials and Methods: We exposed monolayers of HUC (well characterized for their proliferation, qualitative evaluation and quantitative measurement of cytokeratins) to either pure or diluted liquid latex extracts, obtained under standard conditions or by direct contact with materials.Results: The latex urinary catheter appears to be highly toxic since cell viability and metabolic activity were about 10% of those of negative controls for original extracts. Concerning direct contact, latex reduced cell viability, metabolic activity and cell proliferation of HUC on days 1, 3 and 8.Conclusion: The high toxicity of latex on HUC is confirmed for extracts and direct contact. Therefore, it should no longer be used for urinary catheters.

Keywords:
Urothelial cell culture, Biocompatibility, Latex, Cytotoxicity
1.
Mardis HK, Kroeger RM: Ureteral stents: Use and complications. Probl Urol 1992;6:296– 306.
2.
Ruutu M, Alfthan O, Talja M, Andersson C: Cytotoxicity of latex urinary catheters. Br J Urol 1985;57:82–87.
3.
Smith JM, Niligan M: Urethral strictures after open–heart surgery. Lancet 1982;i:392.
4.
Sutherland PD, Maddern PJ, Jose JS: Urethral strictures after cardiac surgery. Br J Urol 1983;55:413–416.
5.
Talja M, Andersson LC, Ruutu M, Alfthan OS: Toxicity testing of urinary catheters. Br J Urol 1985;57:579–584.
6.
Graham DT, Mark GE, Macarthur EB, Pomeroy AR: In vivo validation of a cell culture test for bicompatibility testing of urinary catheters. J Biomed Mater Res 1984;18:1125– 1135.
7.
Harmand MF: Cytotoxicity, toxicological risk evaluation using cell culture; in Braybrook JH (ed): Biocompatibility Assessment of Medical Devices and Materials. New York, Wiley, 1997, pp 119–128.
8.
ISO10993–5: Biological Evaluation of Medical Devices. Test for Cytotoxicity: In vitro Methods. CEN, Geneva, Switzerland, 1992.
9.
Standard Practice for Direct Contact Cell Culture Evaluation of Materials for Medical Devices. Annual Book of ASTM Standards, Medical Devices and Services Section 13–F813–83, 277279, ASTM, Philadelphia, 1991.
10.
Mosmann T: Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55–63.
11.
Sgouras D, Duncan R: Methods for the evaluation of biocompatibility of soluble synthetic polymers which have potential for biomedical use: 1 Use of the tetrazolium–based colorimetric assay (MTT) as a preliminary screen for evaluation of in vitro cytotoxicity. J Mater Sci Mater Med 1990;1:61–68.
12.
Parish CR, Mullbacher A: Automated colorimetric assay for T–cell cytotoxicity. J Immunol Methods 1983;58:225–237.
13.
Hauschka PV, Mavrakos AE, Iafrati MD, Doleman SE, Klagsbrun SE: Growth factors in bone matrix. J Biol Chem 1986;261:12665– 12674.
14.
Cordonnier JJ, Roane JS: Uretral splinting. Surg Clin North Am 1950;58:70–72.
15.
Keitzer WA, Abreu A, Navarro I, Bernreuter E, Allenj S: Urethral strictures: Prevention with plastic indwelling catheters. J Urol 1968;99: 187–188.
2000
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