...Tomokazu Takahashi; Masato Yoshioka; Hiroshi Uchinami; Yasuhiko Nakagawa; Naohiko Otsuka; Satoru Motoyama; Yuzo Yamamoto Purpose: The involvement of hepatic stellate cells (HSCs) with ischemia-reperfusion (I/R) injury in rat liver was examined using gliotoxin, which is known to induce HSC apoptosis...

... for injection into the hepatic artery in mice. The technique was recently used for analysis of the phenomenon of endothelial capture in liver tumors. Methods: Two different models of hepatic tumors in C57BL/6 mice were used. Tumors were induced by intrahepatic cell inoculation. The preferential blood supply...

... (VSOP) supplemented with nitric oxide (NO) gas during the 1st and 24th hour of cold storage (CS) in warm ischemia (WI)-damaged experimental liver grafts. Materials and Methods: Liver grafts (n = 5) were retrieved after 30 min of WI induced by cardiac arrest and CS in histidine-tryptophan-ketoglutarate...

... to damaged cell membranes initiates complement activation and aggravates I/R injury in various organs. However, the time course of CRP- and IgM-mediated complement activation and the relation to hepatocellular injury and inflammation in liver I/R are unknown. Aim: To evaluate the time course of IgM- and CRP...

...Yoshifumi Matsuura; Hiroshi Yagi; Sachiko Matsuda; Osamu Itano; Koichi Aiura; Shun’ichi Kuroda; Masakazu Ueda; Yuko Kitagawa For the targeted delivery of genes and drugs to the human liver, hepatitis B virus (HBV) envelope L particles, which form hollow nanoparticles and display a peptide...

... of the present study was to analyze liver blood flow heterogeneity in detail using LDF devices under normal and low-flow conditions. Materials and Methods: In 5 anesthetized and laparotomized Suabian-Hall strain pigs, systemic hemodynamics and hepatic arterial/portal venous blood flow were constantly recorded...

...O. Grottke; T. Braunschweig; B. Philippen; K.H. Gatzweiler; N. Gronloh; M. Staat; R. Rossaint; R. Tolba Background: To elaborate the impact of new haemostatic agents we developed an instrument for the pressure-controlled induction of blunt liver injuries in a porcine animal model. Materials...

...G. Pestel; K. Fukui; H. Hager; A. Kurz; L. Hiltebrand Background: Difference in pulse pressure (dPP) confirms adequate intravascular filling as a prerequisite for tissue perfusion. We hypothesized that both oxygen and dobutamine increase liver tissue oxygen tension (pt O 2 ). Methods: Eight...

...B. Li; A. Saliez; L. Lambotte The ligation of portal branches is known to induce an atrophy of the deprived lobes and a hypertrophy of the nonligated lobes. In this work, we examined if this response occurs when the ligation affects 90% of the liver mass and if it is able to influence the survival...

...B. Ohlsson; J. Axelson; J.F. Rehfeld; I. Ihse Cholecystokinin (CCK) is a potent stimulus of pancreatic enzyme secretion and growth and is known to influence the flow of biliary secretions. It has also been suggested as a trophic stimulus of the biliary tract and liver, but confirmatory studies...

...G. Zavagno; D. Vespa; Nicoletta Moschin; C. Belluco; A. Cecchetto; Elisabetta Bertocco; A. Raimondo; M. Lise Four groups of isolated rat livers underwent perfusion at 37 °C for 1 h, at 37, 40, 43 or 45 °C, respectively, during the 2nd hour and then at 37 °C again for the 3rd hour. Vascular...

...Y. Yamamoto; M. Obara; W. Isselhard; J. Sturz; T. Morimoto; T. Minor; S. Saad; K. Ozawa In liver transplantation, graft viability is ideally to be determined before implantation. Integrity of mitochondria may be a prerequisite to a viable graft. A new method is presented, which allows...

...T. Minor; W. Isselhard This study was undertaken in order to assess the role of purely circulation-related effects upon free-radical-mediated reperfusion injury in the liver by comparing the respective effects of the oxygen free-radical scavenger superoxide dismutase (SOD) and the vasodilative...

...K. Kuroe; T. Kurokawa; M. Nishikimi; T. Nonami; A. Harada; A. Nakao; H. Takagi This study was designed to clarify the mechanism of a reversal of the ischemia-induced decrease in adenosine triphosphate (ATP) in relation to the changes in liver blood flow. All vessels to the left and median lobes...

...P. Romagnoli; S. Rossi; F. Filipponi; M. Marsili; S. Boncinelli The fine structure of the pig liver was examined after 30 min of warm ischemia at 37 °C, after 2 h of cold ischemia at 4 °C and after 30 min of warm ischemia followed by a further 30 min of cold ischemia. After warm ischemia, limited...

...W. Karwinski; A. Drange; M. Farstad; R. Ulvik; O. Søreide The effect of allopurinol was studied in a normothermic liver ischemia rat model. Functional (bile flow) and biochemical parameters (high-energy phosphates, ATP, ADP, AMP), energy charge, hypoxanthine and xanthine were determined prior...

...T. Minor; C.W. Chung; Y. Yamamoto; M. Obara; S. Saad; W. Isselhard In order to investigate the effects of the exogenously administered radical scavenger superoxide dismutase (SOD) on the orthotopic liver graft, livers from male Wistar rats were transplanted after subjection to 40 min of warm...

...W. Karwinski; M. Farstad; R. Ulvik; O. Sreide A rat liver normothermic ischemia-reperfusion model was used to investigate whether hypoxanthine (HXA) that accumulated during ischemia was washed out in hepatic veins during reperfusion. Ischemia was induced in median and left lobes by clamping...

... = 11) showed about the same unspecific histological shock features. In the liver, however, we diagnosed partly disseminating, partly confluent, but obviously ET-induced, neutrophil liver cell necrosis in 10/12 ETT pigs and in 10/11 controls. We conclude that ETT in our model was not a protective factor...

..., experiments were performed on isolated perfused dog livers, allowing a comparison of the consequence of swelling induced by a hypotonic perfusate with these of anoxia or of anoxia combined with swelling. Swelling alone imposed during 4 h results, after correction of the osmolarity, in a reduction of cell...