Background/Aims: The present study examined the preventive effect of ginger against renal ischemia-reperfusion (I-R) injury. Methods: 30 adultmale rats were used. The animals were allocated to five groups of 6 rats: (1) normal (N), (2) normal + ginger (N+G), (3) right nephrectomy (Sham), (4) I-R, and (5) I-R + ginger (I-R+G). To induce renal ischemia, animals were unilaterally nephrectomized and subjected to 45 min of left renal pedicle occlusion with 24 h reperfusion. Ginger was administered orally 24 h and just before ischemia. At the end of the experiment, blood samples and urine were collected. The following renal functional parameters were studied: serum and urinary levels of creatinine and urea, fractional excretion of sodium, and creatinine clearance. Histopathological examination of the kidney was also performed. Results: Rats with renal I-R showed a significant increase in creatinine and urea concentration in serum and also a significant decrease in creatinine clearance compared with the other groups. In addition, renal histopathology in the I-R group showed severe alterations such as tubular dilatation and tubular epithelial necrosis. However, these changes were significantly reduced in the I-R+G group. Conclusion: This study suggests that ginger is a useful agent for the prevention of renal ischemia reperfusion-induced injury.

Keywords:
Ginger, Ischemia-reperfusion, Renal function
1.
Shingu C, Koga H, Hagiwara S, Matsumoto S, Goto K, Yokoi I, Noguchi T: Hydrogen-rich saline solution attenuates renal ischemia-reperfusion injury. J Anesth 2010;24:569–574.
2.
Abdollahi M, Ranjbar R, Shadnia S, Nikfar S, Rezaie A: Pesticides and oxidative stress: a review. Med Sci Monit 2004;10:141–147.
3.
Thadhani R, Pascual M, Bonventre JV: Acute renal failure. N Engl J Med 1996;334:1448–1460.
4.
Bulkley GB: Reactive oxygen metabolites and reperfusion injury: aberrant triggering of reticuloendothelial function. Lancet 1994;344:934–935.
5.
Yoshioka T, Ichikawa I: Glomerular dysfunction induced by polymorphonuclear leukocyte-derived reactive species. Am J Physiol 1989;257:53–59.
6.
Bird JE, Milhoan K, Wilson CB, Young SG, Mundy CA, Parthasarathy S, Blantz RC: Ischemic acute renal failure and antioxidant therapy in the rat: the relation between glomerular and tubular dysfunction. J Clin Invest 1987;81:1630–1638.
7.
Paller MS: Renal work, glutathione and susceptibility to free radical-mediated post-ischemic injury. Kidney Int 1988;33:843–849.
8.
Burns AT, Davies DR, McLaren AJ, Cerundolo L, Morris PJ, Fuggle SV: Apoptosis in ischemia/reperfusion injury of human renal allografts. Transplant 1998;66:872–876.
9.
Lee SJ, Kyoung Y, Na KR, Lee KW, Suh KS: The preconditioning with AICAR protects against subsequent renal ischemia reperfusion injury. Korean J Nephrol 2000;28:96–102.
10.
Venardo KM, Kaye DM: Myocardial ischemia-reperfusion injury, antioxidant enzyme systems, and selenium: a review. Curr Med Chem 2007;14:1539–1549.
11.
Cuzznocrea S, Riley DP, Caputi AP, Salvemini D: Antioxidant therapy: a new pharmacological approach in shock, inflammation, and ischemia/reperfusion injury. Pharmacol Rev 2001;53:135–159.
12.
Riksen NP, Oven WJ, Ramkers BP, Van de Broek PH, Engbersen R, Boerman OC, Smits P, Rongen GA: Oral therapy with dipyridamole limits ischemia reperfusion injury in humans. Clin Pharmacol Ther 2005;78:52–59.
13.
Taniguchi M, Magata S, Suzuki T, Shimamura T, Jin MB, Iida J, Furukawa H, Todo S: Dipyridamole protects the liver against warm ischemia and reperfusion injury. J Am Coll Surg 2004;198:758–769.
14.
Sadis C, Teske G, Stokman G, Kubjak C, Claessen N, Moore F, Loi P, Daillo B, Barvais L, Goldman M, Florquin F, Moine A: Nicotine protects kidney from renal ischemia/reperfusion injury through the cholinergic anti-inflammatory pathway. PLoS ONE 2007;2:e469.
15.
Wagner K, Albrecht S, Neumayer HH: Prevention of post transplant acute tubular necrosis by calcium antagonist diltiazem: a prospective randomized study. Am J Nephrol 1987;7:287–291.
16.
Tucci S Jr, Borelli-Bovo TJ, Cologna AJ, Tiraboschi RB, Martins ACP, Roselino JR: Calcium channel blocker and renal mitochondrial function in warm renal ischemia. Int Braz J Urol 2005;31:384–389.
17.
Anderson CD, Pierce J, Nicoud I, Belous A, Knox CD, Chari RS: Modulation of mitochondrial calcium management attenuates hepatic warm ischemia reperfusion injury. Liver Transpl 2005;11:663–668.
18.
Kondo N, Kiyomoto H, Yamamoto T, Miyatake A, Sun GP, Rahman M, Hitomi H, Moriwaki K, Hara T, Kimura S, Abe Y, Kohno M, Nishiyama A: Effects of calcium channel blockade on angiotensin II-induced peritubular ischemia in rats. J Pharmacol Exp Ther 2006;316:1047–1052.
19.
Sies H: Strategies of antioxidant defense. Eur J Biochem 1993;215:213–219.
20.
Ghahreman A: Iran’s Cromophytes (Plant Systematic). Tehran, Center of University Publications, 1995, vol 4, pp 516–521.
21.
Lako J, Trenerry C, Wahlqvist ML, Wattanapenpaiboon N, Sotheeswaran S, Premier R: Total antioxidant capacity and selected flavonols and carotenoids of some Australian and Fijian fruits and vegetables. Asia Pac J Clin Nutr 2004;13(suppl):S127.
22.
Thomson M, Al-Qattan KK, Al-Sawan SM, Alnaqeeb MA, Khan I, Ali M: The use of ginger (Zingiber officinale Rosc.) as a potential anti-inflammatory and antithrombotic agent. Prostaglandins Leukot Essent Fatty Acids 2002;67:475–478.
23.
Akoachere JF, Ndip RN, Chenwi EB, Ndip LM, Njock TE, Anong DN: Antibacterial effect of Zingiber officinale and Garcinia kola on respiratory tract pathogens. East Afr Med J 2002;79:588–592.
24.
Ficker C, Arnason JT, Vindas PS, Alvarez LP, Akpagana K, Gbeassor M, De Souza C, Smith ML: Inhibition of human pathogenic fungi by ethnobotanically selected plant extracts. Mycoses 2003;46:29–37.
25.
Halvorsen BL, Holte K, Myhrstad MC, Barikmo I, Hvattum E, et al: A systematic screening of total antioxidants in dietary plants. J Nutr 2002;132:461–471.
26.
Thiemermann C, Patel NSA, Kvale EO, Cockerill GW, Brown PAJ, Stewart KN, Cuzzocrea S, Britti D, Mota-Filipe H, Chatterjee PK: High-density lipoprotein reduces renal ischemia/reperfusion injury. J Am Soc Nephrol 2003;14:1833–1843.
27.
Kurata H, Fujii T, Tsutsui H, Katayama T, Ohkita M, Takaoka M, Tsuruoka N, Kiso Y, Ohno Y, Fujisawa Y, Shokoji T, Nishiyama A, Abe Y, Matsumura Y: Renoprotective effect of L-carnosine on ischemia/reperfusion-induced renal injury in rats. J Pharmacol Exp Ther 2006;319:640–647.
28.
Chen H, Xing B, Liu X, Zhan B, Zhou J, Zhu H, Chen Z: Ozone oxidative preconditioning inhibits inflammation and apoptosis in a rat model of renal ischemia/reperfusion injury. Eur J Pharmacol 2008;581:306–314.
29.
Barnes JL, Osgood RW, Reineck HJ, Stein JH: Glomerular alterations in an ischemic model of acute renal failure. Lab Invest 1981;45:378–386.
30.
Finn WF: Nephron heterogeneity in polyuric acute renal failure. J Lab Clin Med 1981;98:21–29.
31.
Tanner GA, Steinhausen M: Tubular obstruction in ischemia-induced acute renal failure in the rat. Kidney Int Suppl 1976;6:S65–S67.
32.
Krishan P, Sharma A, Singh M: Effect of angiotensin converting enzyme inhibitors on ischemia-reperfusion-induced renal injury in rats. J Pharmacol Exp Ther 1994;27:769–775.
33.
Williams RH, Thomas CE, Navar LG, Evan AP: Hemodynamic and single nephron function during the maintenance phase of ischemic acute renal failure in the dog. Kidney Int 1981;19:503–515.
34.
Kang DG, Sohn EJ, Moon MK, Lee YM, Lee HS: Rehmannia glutinosa ameliorates renal function in the ischemia/reperfusion-induced acute renal failure rats. Biol Pharm Bull 2005;28:1662–1667.
35.
Weinberg JM: The cell biology of ischemic renal injury. Kidney Int 1991;39:476–500.
36.
Rabb H, Omeara YM, Coleman P, Brady HR: Leukocytes, cell adhesion molecules and ischemic acute renal failure. Kidney Int 1976;51:1463–1468.
37.
Williams P, Lopez LL, Britt D, Chan C, Ezrin A, Hottendorf R: Characterization of renal ischemia-reperfusion injury in rats. J Pharmacol Toxicol Methods 1997;37:1–7.
38.
Chander V, Chopra K: Renal protective effect of molsidomine and L-arginine in ischemia-reperfusion induced injury in rats. J Surg Res 2005;128:132–113.
39.
Hearse DJ: Prospects for antioxidant therapy in cardiovascular medicine. Am J Med 1991;91:118S–121S.
40.
Ahmed RS, Seth V, Banerjee BD: Influence of dietary ginger (Zingiber officinale Rosc.) on antioxidant defence system in rat: comparison with ascorbic acid. Indian J Exp Biol 2000;38:604–606.
41.
Uz E, Karatas OF, Mete E, Bayrak R, Bayrak O, Atmaca AF, Atis O, Yildirm ME, Akcay A: The effect of dietary ginger (Zingiber officinale Rosc.) on renal ischemia/reperfusion injury in rat kidneys. Renal Fail 2009;31:251–260.
42.
Hudson EA, Fox LH, Luckett JCA, Manson MM: Ex vivo cancer chemoprevention research possibilities. Environ Toxicol Pharmacol 2006;21:204–214.
43.
Ajith TA, Nivitha V, Usha S: Zingiber officinale Roscoe alone and in combination with α-tocopherol protect the kidney against cisplatin-induced acute renal failure. Food Chem Toxicol 2007;45:921.
44.
Toda S, Kumura M, Ohnishi M: Effect of phenolcarboxylic acids on superoxide anion and lipid peroxidation induced by superoxide anion. Planta Med 1991;57:8.
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