With a 2.9-mM concentration of unlabelled bovine serum albumin (BSA), the FITC-albumin transport (2 mg included) across the omental monolayer (0.48 ± 0.16 mg/ml/30 min) was found to be significantly reduced as compared with the interstitial BSA concentration (290 µM) as it is the case, e.g., in peritonitis (0.79 ± 0.09 mg/ml/30 min). Adding 10 µg lipopolysaccharide (LPS)/ml from Escherichia coli, serotype 0128:B12, we did not see any differences from the control. Cultured mesothelial cells took up double the amount of FITC-albumin (4.2 ± 0.13 µg/105 cells/30 min) and in the presence of LPS the uptake of FITC-albumin was reduced to half the control (2.15 ± 0.47 µg/105 cells/30 min). The results reveal the active participation of the mesothelium because high concentrations of BSA reduced exocytosis and stimulated endocytosis. Applying 10 µg/ml of LPS turned out to influence endocytosis and to reduce it at a high BSA concentration.

Keywords:
Mesothelial cells, Peritoneal cavity, Albumin transcytosis, Permeability, albumin
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© 1991 S. Karger AG, Basel
1991
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