... and repeated isozyme evolution, a subject where alcohol dehydrogenases exceptionally well offer detailed examples. Human aldehyde dehydrogenase occurs of two types, a mitochondrial and a cytosolic form. The enzymes are tetramers, do not contain functional metals, and have subunits which do not form inter-type...

... and is stimulated by Mg^2+, the other is operative with millimolar acetaldehyde concentrations and is stimulated by adenosine 5'-triphosphate (ATP). The third system reduces added NAD and is stimulated by rotenone. Connected to these systems, three aldehyde dehydrogenase isozymes (ALDH) have been purified: a low-K...

...D. Meier-Tackmann; D. P. Agarwal; N. Saha; H. W. Goedde Human stomach aldehyde dehydrogenase (ALDH) isozymes were screened in random autopsy specimens from 66 North German and 33 Chinese individuals. Three ALDH isozymes were identified, which differed in their electrophoretic mobility, affinity...

...D. Meier-Tackmann; G.C. Korenke; D.P. Agarwal; H.W. Goedde Freshly obtained human term placentae were subjected to subcellular fractionation to study the localization of NAD-dependent aldehyde dehydrogenases. Optimal conditions for the cross-contamination-free subcellular fractionation were...

...H. Werner Goedde; Dharam P. Agarwal; Shoji Harada Human liver alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH)play an important role in alcohol metabolism. It has been shown that both enzymes are polymorphic and that different variants may be responsible for individual and racial...

...Fritz Heinz; Friederike Schlegel; Peter H. Krause Activities of enzymes involved in fructose metabolism were measured in samples of human kidney cortex and medulla. The enzymes are ketohexokinase, aldolase,NAD- and NADP-dependent alcohol dehydrogenase, aldehyde dehydrogenase, triokinase...

... region and ileum in the region of the valvula ileo coecalis. Ketohexokinase,aldolase, alcohol dehydrogenases NAD- and NADPdependent were found in all gut sections. The activity of aldehyde dehydrogenase was low in all sections tested. Triokinase could be found only in the duodenum and jejunum region...

...Yoshikazu Aoki; Hajime Itoh The effects of long-term and short-term exposure of rats to ethanol on aldehyde dehydrogenase (ALDH) activity in the liver mitochondria were investigated. The specific activities of mitochondrial high Km ALDH and low Km ALDH after the prolonged administration of ethanol...

...Dharam Agarwal; Patricia Cohn; Werner Goedde; John Hempel Human red cell aldehyde dehydrogenase (ALDH) resembles the liver cytosolic isozyme in numerous physicochemical properties. This study was undertaken to establish the structural relationship between the erythrocyte and liver ALDH isozymes...

...M.J.C. Crabbe; S.T. Hoe Human lens was found to contain aldehyde dehydrogenase at a level of activity similar to that of bovine lens, namely 1.76 ± 0.51 IU/g. The enzyme, which appears to be a tetramer of 229 kD, was less susceptible to inhibition by cataractogenic agents than the bovine enzyme...

...Akira Yoshida; Lily C. Hsu; Vibha Davé The major cytosolic aldehyde dehydrogenase isozyme (ALDH1) exhibits strong activity for oxidation of retinal to retinoic acid, while the major mitochondrial ALDH2 and the stomach cytosolic ALDH3 have no such activity. The K(m) of ALDH1 for retinal is about...