... to in the content or advertisements. Marker enzymes Plasma membranes Subcellular distribution Isoenzymes Adenyl cyclase 5'-Nucleotidase ATPases Phosphomonoester hydrolases Aminopeptidases Short Review Enzyme 13: 329-372 (1972) Enzyme Markers in Characterization of Isolated Plasma Membranes...

...Krystyna Konopka; Maria Gross-Bellard; W. Turski The activation of 5'-nucleotidase in the homogenate as well as in the subcellular fractions, is due to the action of sodium deoxycholate and Triton X-100. Beyond this, both detergents cause a partial solubilization of the enzyme. The solubilized part...

...D.D. Jones; G.F. Williams; B. Prochazka An attempt has been made to show that the increase in enzyme activities in sera of pregnant women found with glucose-6-phosphate and adenosine 5'-monophosphate as substrates (described as glucose-6-phosphatase and 5'-nucleotidase)was due to the increase...

...Michel Le Hir An impurity, probably an anion, present in some batches of the buffer substances 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES), 2-morpholinoethane sulfonic acid (Mes) and piperazine-l,4-bis(2-ethane sulfonic acid (Pipes), activates the soluble 5'-nucleotidase from rat...

...J.M. Gandarias; J.J. Goiriena; E. Rueda; B.M. Fernandez 5'-Nucleotidase was measured in isolated fat cells from normal, hypothyroid and hyperthyroid rats. This was done to find out whether thyroid hormones had an effect on the production of adenosine by the fat cell. The results showed that 5...

...George A.J. Goodlad; Catherine M. Clark The effect of the growth of the Walker 256 carcinoma on the level of 5'-nucleotidase and alkaline phosphatase in the whole liver and in an isolated hepatocyte membrane preparation of its host was investigated. Alkaline phosphatase activities of whole liver...

...Mark C. Lakshmanan; Myong Won Kahng; Raymond T. Jones The plasma membrane enzymes, alkaline phosphatase, bicarbonate-dependent adenosine triphosphatase, 5'-nucleotidase, and carbonate dehydratase, were measured in ductal and acinar preparations of bovine pancreas. Epithelial cells were scraped from...

...Janusz Greger; Krystyna Fabianowska-Mafewska The activities of 5'-nucleotidase (5'-ribonucleoside phosphohydrolase, EC 3.1.3.5);adenosine deaminase (adenosine aminohydrolase, EC 3.5.4.4); AMP deaminase (AMP aminohydrolase,EC 3.53.6), and ATP-(Mg^2+)-ase (ATP phosphohydrolase, EC 3.6.1.3) were...

...Hedwig Bodanszky; Olga Greengard; Tiberius Den The lung of adult man contains several times more 5'-nucleotidase (EC 3.1.3.5),alkaline phosphatase (EC 3.1.3.1) and γ-glutamyl-transpeptidase (EC 2.3.2.2) per gram than does that of the 12- to 16-week fetus. In rat lung, too, there is a drastic...

...Peter W. Andrew; Douglas B. Lowrie; Timothy J. Peters 5'-Nucleotidase has been demonstrated in rabbit alveolar macrophages. This enzyme has been found to be localised on the plasma membrane as have alkaline phosphodiesterase I and leucyl-2-naphthylamidase. Heterogeneity of the plasma membrane has...

...-Glutamyltransferase 5'-Nucleotidase Peripheral blood mononuclear cells Subcellular localization Enzyme 1989;41:17-26 1607100 © 1989 S. Karger AG, Basel 0013-9432/89/0411-0017S2.75/0 Subcellular Localization and Kinetic Properties of Phosphatidylinositol 4,5-Bisphosphate Phospholipase C and Inositol Phosphate...

...Michel Le Hir; Ragini Gandhi; Ulrich C. Dubach 5'-Nucleotidase activity was solubilized from a particulate fraction of rat renal homogenates by Sulphobetaine 14. An 11,430-fold purification was achieved by a two-step chromatographic procedure using concanavalin-A Sepharose and ADP-agarose. SDS-PAGE...

...S. Halsall; T. J. Peters After 6 weeks of chronic ethanol consumption hepatic γ-glutamyl-transferase and -hydrolase activities increased compared with pair-fed controls. There was no change in 5'-nucleotidase activity. It was found that the increase in γ-glutamyltransferase activity occurred...