Lactate dehydrogenase isoenzymes were investigated in supernatants of small-intestinal mucosa of 24 men and 18 females with the method of electrophoresis in agar gel and tétrazolium staining. In nontropical sprue and children with celiac disease the most positively charged fraction (LDH 5) was always decreased with simultaneous elevations of LDH 4, LDH 3, the ratio (LDH 3 + LDH 4) : LDH 5 and the value of H subunits. These changes were significant in comparison with controls and patients with secondary malabsorption of small-intestinal as well as pancreatic origin. In advanced exocrine pancreatic insufficiency changes significant in comparison with controls were also observed. The distribution of LDH isoenzymes in ileal adenocarcinoma resembled more closely the pattern of primary malabsorption than any other condition examined. The pattern of LDH isoenzymes of small intestinal mucosa in primary malabsorption approximates the distribution found in human foetal tissues. The evidence of identity of nontropical sprue and celiac disease of children at the molecular evel of the cell is presented. The relative activities of genes controlling the synthesis of LDH monomers in small-intestinal mucosa appear to be altered in favor of the gene controlling the synthesis of H subunits. LDH isoenzymes of jejunal supernatants in advanced pancreatic insufficiency may be influenced by various factors and more definite explanation of the observed changes cannot be given on the basis of the present material. The results are discussed in view of their possible significance for future studies on pathophysiology and heredity of primary malabsorption.

This content is only available via PDF.
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
1966
You do not currently have access to this content.