The biochemical triad, NADH oxidation, oxygen (0(2)) uptake and hydrogen peroxide(H(2)0(2)) formation, by subcellular fractions of human blood polymorphonuclears (PMNs) was investigated. It was found that this biochemical triad (1) was under the control of the granule-rich fraction (GRF) only; (2) was not inhibited by cyanide; (3) occurred stoichiometrically for its three components, and (4) accounted quantitatively for the respiratory burst of the stimulated PMN. It was also shown that the above biochemical triad (1) involved an enzymatic step; (2) was enhanced by acidic pH (5.0) and Mg^++; (3) was inhibited by Cu^++ or low concentration of Mn^++;(4) was dependent on H(2)0(2), perhydroxyl radical (HO(2)) and hydroxyl radical (HO•) since either catalase or superoxide dismutase or scavengers of HO•(2) or HO. were inhibitor, and (5) involved multistep reactions. Evidence is provided that the sequence of the reactions is first a generation of H(2)0(2) (spontaneously from NADH in our incubation medium), secondly the production of HO•from H(2)0(2), thirdly the oxidation of NADH with further production of HO•(2), 02 uptake and H(2)0(2)formation, probably through a chain reaction. The identification of the enzyme(s) involved in these multistep reactions needs further studies.

This content is only available via PDF.
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.