Inactivation of rat liver cystathionase was studied in homogenates made in buffers at various pH values and incubated at 37 °C for various lenghts of time, and also in supernatants resulting from ultracentrifugation of homogenates prepared in 0.25 mol/l saccharose, and eventually adjusted at definite pH prior to incubation. The stability of the enzyme in homogenates varies extensively depending upon pH values: acidic conditions favour inactivation. In supernatants, whatever the pH, the stability of the enzyme was greater than in corresponding homogenates. These results suggest that hydrolytic enzymes present in particulate fractions (quite likely lysosomes) take part in the inactivation of cystathionase in the liver. In addition, since we observed that pyridoxal phosphate added to the enzymic preparations prior to incubation protected the enzyme against inactivation, this observation affords new evidences that holocystathionase is more stable than the apoenzyme.

This content is only available via PDF.
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.