The reaction catalyzed by the glucose oxidase (EC 1.1.3.4) from Aspergillus niger was markedly inhibited by Ag+,Hg^++, Cu^++, PCMB and mercuric sulfadiazine, while the silver antimicrobials,silver sulfadiazine, and silver uracil had no inhibitory effect. Characteristic difference spectra due to the absorption by the FAD moiety of the enzyme were obtained by the addition of Ag^+, Hg^++, Cu^++, PCMB and mercuric sulfadiazine, while no spectral changes were seen on the addition of silver sulfadiazine and various silver uracil compounds. Using the ‘flow method’ to study overall kinetics, it was determined that mercuric sulfadiazine inhibited the reduction of the FAD moiety, thereby competing with the molecular oxygen used as a hydrogen acceptor.

Keywords:
Glucose oxidase, Silver uracils, Metallic ions, Flow method, Aspergillus niger, Silver sulfadiazine, Mercuric sulfadiazine
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1972
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