The fate of 14C-LDH5 after intravenous and intraperitoneal injection was studied in dogs and rats. The enzyme was labelled by means of incorporating 14C-acetate groups into the enzyme protein. The turnover of extracellular 14C-LDH5 was investigated by measurement of 14C-radioactivity, enzyme activity,and by autoradiography. The elimination of 14C-LDH5 takes place in the intravascular space, as well as in the extravascular fluid compartments. Part of the 14C-LDH(5)-radioactivity is excreted in urine,bile, and the gastrointestinal tract; a smaller amount remains in the organism. A specific elimination organ cannot be distinguished, as the enzyme elimination is multilocal. The significance of enzyme elimination in the diagnostic assessment of enzyme levels in serum is indicated. The labeling of enzymes with isotopes and the behavior of cell enzymes in the extracellular compartment are discussed, and the results are compared with other results in the literature.

Keywords:
Radioactive-labelled enzymes, Homologous and heterologous, Enzyme elimination, Distribution and half-life of enzymes in body fluids
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1972
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