A cDNA encoding epitopes present in paired helical filaments (PHF) in Alzheimer's disease has been characterized by DNA sequencing and by immunochemical analysis of the encoded recombinant protein fragment. The cDNA encodes 773 amino acids specific of the rat high molecular weight microtubule-associated protein MAP2. This amino acid sequence is localized in the side arm projection domain of MAP2. An antiserum raised against the expressed fusion protein encoded by this cDNA labels the high molecular weight MAP2 but not the 70 kDa MAP2c abundant in developing brain. This antiserum labels PHF in situ but only a proportion of isolated PHF. In situ hybridization on human brain sections did not demonstrate an increase in mRNA for MAP2 in tangle-bearing neurones. We suggest that the association of this MAP2 fragment with PHF in situ results from posttranslational modifications of MAP2.

This content is only available via PDF.
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.