Background: Retinaldehyde has been shown to exert antibacterial activity in vitro. Aim: This study evaluates the effect of retinaldehyde on Propionibacterium acnes both in vivo and in vitro. Methods: Microbial minimal inhibitory concentrations (MICs) of retinaldehyde and retinoic acid were determined on reference strains of P. acnes. In vivo activity of daily topical application of 0.05% retinaldehyde on the P. acnes density was evaluated after application in a single-blind randomised study. Results: MICs of retinaldehyde were 4 mg/l for P. acnes No. CIP179 and CIP53119 and 8 mg/l for P. acnes No. CIP53117. In contrast, the MICs of retinoic acid were superior to 128 mg/l for these three strains. In vivo, retinaldehyde-treated areas displayed a significant decrease in counts of viable P. acnes as compared with the untreated areas with a median decrease of 102 log P. acnes/cm2 after 2 weeks of daily application. Vehicle alone had no effect. Conclusion: The MIC of retinaldehyde against P. acnes suggests a direct antibacterial activity. Daily topical application of 0.05% retinaldehyde is associated with a clear reduction of the P. acnes density.

Keywords:
Topical retinaldehyde, Propionibacterium acnes
1.
Green HN, Mellanby E: Vitamin A as an anti-infective agent. Br Med J 1928;ii:691–696.
2.
Cohen BE, Elin RJ: Vitamin A induced nonspecific resistance to infection. J Infect Dis 1974; 129:597–600.
3.
Krishnan S, Krishnan AD, Mustapha AS, Talwar GP, Ramalingaswami V: Effect of vitamin A and undernutrition on the susceptibility of rodents to a malarial parasite, plasmodium berghei. J Nutr 1976;106:784–791.
4.
Bang FB, Bang BG, Foard M: Acute Newcastle disease virus infection of the upper respiratory tract of the chicken. The effect of diets deficient in vitamin A on the pathogenesis of the infection. Am J Pathol 1975;79:417–424.
5.
Nauss KM, Anderson CA, Connors MW, Newberne PM: Ocular infection with herpes simplex virus (HSV-1) infection. J Nutr 1985;115: 1300–1315.
6.
Ross AC, Hämmerling UG: Retinoids and the immune system; in Sporn MB, Roberts AB, Goodman DS (eds): The retinoids: Biology, Chemistry and Medecine, ed 2. New York, Raven Press, 1994, pp 597–630.
7.
Pechère M, Siegenthaler G, Pechère JL, Saurat J-H: Antibacterial effect of retinaldehyde and derivatives (abstract). J Invest Dermatol 1996; 106:912.
8.
Leyden JJ, McGinley KJ, Nordstrom KM, Webster GF: Skin microflora. J Invest Dermatol 1987;88:S65–S71.
9.
National Comittee for Clinical Laboratory Standards: Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, ed 2, approved standard. Villanova, NCCLS, 1990, vol 8.
10.
Williamson P, Kligman AM: A new method for the quantitative investigation of cutaneous bacteria. J Invest Dermatol 1965;45:498–530.
11.
Peck GL, Olsen TG, Yoder FW, Strauss JS, Downing DT, Pandya M, Butkus D, Arnaud-Batendier J: Prolonged remission of cystic and conglomata acne with 13-cis-retinoic acid. N Engl J Med 1979;300:329–333.
12.
Plewig G, Nikolowski J, Wolff HH: Action of isotretinoin in acne rosacea and gram-negative folliculitis. J Am Acad Dermatol 1983;6:766– 785.
13.
Leyden JJ, McGinley KJ, Foglia AN: Qualitative and quantitative changes in cutaneous bacteria associated with systemic isotretinoin therapy for acne conglobata. J Invest Dermatol 1986;86:390–393.
14.
Weissmann A, Wagner A, Plewig G: Reduction of bacterial skin flora during oral treatment of severe acne with 13-cis-retinoic acid. Arch Dermatol Res 1981;270:179–183.
15.
Simjee S, Sahm DF, Soltani K, Morello JA: Organisms associated with gram-negative folliculitis: In vitro growth in the presence of isotretinoin. Arch Dermatol Res 1986;278:314– 316.
16.
Flemetakis AC, Tsambaos DG: Effects of synthetic retinoids on the growth of bacteria and their susceptibility to antibiotics. J Chemother 1989;1:374–376.
17.
McGinley KJ, Webster GF, Leyden JJ: Regional variations of cutaneous propionibacteria. Appl Environ Microbiol 1974;35:62–65.
1999
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