...-exchange chromatography on DEAE-Sephadex A 50 and then on SP-Sephadex C 50. The electrophoresis on SDS-polyacrylamide gels showed only one spot that comigrated with commercial human albumin. Binding to albumin was measured by equilibrium dialysis of an aliquot of albumin solution(0.7 ml) against the same...

... tissues were found. Ouabain binding stability to Na-K ATPase extracted from myo and RBC did not vary with age. Binding studies revealed a K(D) X 10^-8 M of 0.87 ± 0.09 for NB human RBC and 0.88 ± 0.14 for Ad human RBC. Calculated RBC receptor density however, was higher in the Ad (1.25 ± 0.08 sites/μm^2...

...A. Robertson; R. Brodersen Drugs which compete with bilirubin for albumin binding may increase the risk of kemicterus. Fortunately, few drugs are strong competitors. However, in neonatology,many drugs are used simultaneously. We have studied the effect of drug combinations on bilirubin binding...

... and then on SP-Sephadex C 50. The binding of diazepam (1 μM), salicylic acid (2 mM) and digitoxin(6 nM) to albumin (40 g/l) was measured by equilibrium dialysis at 37°C. The unbound fraction (mean ± SD) for fetal and adult albumin of diazepam was 1.86 ± 0.24 and 1.82 ±0.15% (NS), that of digitoxin was 3.18...