The antioxidant properties of alpha-tocopherol and alpha-tocopheryl acetate in lung tissue were quantitated in vitro by measurement of thiobarbituric acid (TBA)reactants, pentane production and lipid peroxides following either an iron/ascorbate or a xanthine/xanthine oxidase oxidant stress. Lung homogenates were obtained from newborn rabbits treated intravenously with either 10 or 100 mg/kg of either alpha-tocopherol or alpha-tocopheryl acetate. The animals were killed 5 min after dosing to minimize the conversion of alpha-tocopheryl acetate to alpha-tocopherol. Lung homogenates from animals treated with alpha-tocopherol had decreased concentrations of TBA reactants and pentane production after incubation with either oxidant stress compared to lung homogenate from animals treated with alpha-tocopheryl acetate or vehicle alone. Lipid peroxide concentrations were no different in homogenates from alpha-tocopherol or alpha-tocopheryl acetate treated animals. Correlations between antioxidant activity and tissue concentrations of alpha-tocopherol indicate 50% inhibition is achieved with 30-100 pg/g lung tissue. In vitro addition of alpha-tocopherol required concentrations between 100 and 200 pg/g to reduce lipid peroxidation by 50%.

Keywords:
Alpha-tocopherol, Alpha-tocopheryl acetate, Antioxidant, Lipid peroxides, Pentane, Thiobarbituric acid reactants, Vitamin E
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1987
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